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Lecture40&41
Edible Vaccines
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EDIBLE VACCINES FROM PLANTS
Two strategies for production
1) Expression of foreign antigens in plant via
stable transformation
2) Delivery of vaccine epitopes via plant virus
(Mason and Arntzen, 1995)
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Strategies for the production of candidate vaccine
antigens in plant tissues
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(Mason and
Arntzen,
1995)
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HEPATITIS B SURFACE ANTIGEN IN TOBACCO
• Gene encoding HBsAg expressed in tobacco
plants
• Targeted to the chloroplast
• Genetic constructs-
1) driven by CaMV 35S promoter
2) 5' UTR from TEV and TMV
3) signal peptide from soybean
VSP
4) A plastid transit peptide
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Contd...
Chloroplast of tobacco
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Contd...
Structures of
expression
cassettes
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• Various expression vectors were constructed
• pHB110 was plastid targeted
• Expression level 0.01% of soluble protein
(Richter et al., 2000)
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RNA Analyzed by Northern Blot Hybridized with
HBsAg coding sequence
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RABIES VIRUS G PROTEIN IN TOMATO
• Gene linked to CaMV35S
promoter
• Introduced to tomato plants
by Agrobacterium- mediated
transformation
• Expression of recombinant
glycoprotein in leaves and
fruits
• Protein localized in golgi
bodies, vesicles and
plasmalemma
(Mc Garvey
et al.,1995)
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Norwalk virus (cold virus) capsid protein in
potato and tobacco
• Causative agent for acute epidemic
gastroenteritis
• NVCP was fused to CaMV35S or patatin
promoter
• Transformation by Agrobacterium
• Expression level:
0.37% TSP in potato tubers
0.23% TSP in tobacco leaves
(Mason
et al.,
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