Transcript File - Sarah johnson

What’s the Hype About Hyperoxaluria?
Kirsten Boestfleisch, Lindsay Edmonds, Lena Husnay, Sarah Johnson
What is Primary Hyperoxaluria?
Diet Implications
More specifically, enzyme deficiencies found in types I and II
lead to the build up of glyoxylate in the body, which is normally oxidized
by lactate dehydrogenase into oxalate. Primary hyperoxaluria type I is
caused by more than 140 mutations in the AGXT gene that lead to the
loss of function in the peroxisomal liver enzyme, alanineglyoxylate
aminotransferase (AGT; Hoppe, 2010). AGT catalyzes the
Figure 1. Glyoxylate Metabolism in the Hepatocyte; Source: Cochat P and Rumsby G, 2013
transamination of glyoxylate to glycine (Raju et al., 2008). Primary
hyperoxaluria type II involves deficiency in the enzyme glyoxylate
reductase-hydroxypruvate reductase (GRHPR; Hoppe et al., 2009). GRHPR is involved in the reduction of two separate
molecules: glyoxylate to glycolate and hydroxypyruvate to D-glycerate.
Primary hyperoxaluria type III was identified in 2010 (Beck et al., 2013) and consists of various sorts of mutations
on chromosome 10 that affect the enzyme 4-hydroxy-2-oxoglutarate aldolase (HOGA1; Belostotsky, 2010). The enzyme,
found in the mitochondria of the liver rather than the cytosol, is involved in catalyzing the
last step in production of glyoxylate and pyruvate from the breakdown of 4-hydroxy-2oxoglutarate. Because type III has been so recently discovered and is found in only about
5% of primary hyperoxaluria cases (Hoppe et al., 2009), no well known mechanisms are
clearly understood in how this enzyme deficiency leads to overproduction of oxalate.
Signs and Symptoms
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Most patients with primary hyperoxaluria experience kidney stones (Beck et al., 2013) which usually consist of
calcium oxalate monohydrate (Cochat et al., 2013). In patients who may experience symptoms of kidney stones,
diagnostic procedures commonly include measurements of urinary levels of oxalate, calcium, citrate, sodium,
magnesium, urate, and also volume and pH of urine (Cochat et al., 2013). End-stage renal disease is also common in
patients with types I and II, however, there are no reported cases in type III so far (Beck et al., 2013).
While primary hyperoxaluria is typically genetic and present throughout most of one’s life, signs may not show
until early or even late adulthood (Fodor et al., 2012). Therefore, the disorder is heavily underdiagnosed and
oftentimes, the first sign of the disorder is renal failure in types I and II (Cochat et
al., 2013; Hoppe et al., 2009). Primary hyperoxaluria causes a poor quality of life
for many patients diagnosed with the disorder. Primary hyperoxaluria is
associated with a high rate of morbidity and mortality. The high death rate
results from the fact that most physicians lack knowledge of primary
hyperoxaluria. Also, the treatment of primary hyperoxaluria requires strict
adherence to conservative treatment and optimal urological
management. Many patients neglect to follow such strict treatments and
therefore experience severe declines in health (Cochat et al., 2013).
http://www.herringlab.com/photos/Urinary/Oxalates/Cod/Cod_B.jpg
Calcium Oxalate Kidney Stone
Restriction of dietary oxalate intake is one of the treatment methods to prevent recurrent nephrolithiasis, as
a result of primary hyperoxaluria in certain patients (Weiwen et al., 2005). Oxalate levels in the body vary greatly
depending on the oxalate content of certain foods consumed in the diet. A food is considered high in oxalates if it has
10 mg of oxalate per serving. A person should limit these high-oxalate foods to no more than two to three servings
per day in order to prevent developing kidney stones. It is also important in conjunction with this diet to drink plenty
of fluids to help flush out the extra oxalate and to avoid high doses of vitamin C supplements, as the body can turn the
extra vitamin C into oxalate (Low Oxalate Diet, 2013).
Fats and nuts high in oxalates include: mixed nuts, nut butter, sesame seeds, tahini, and soy nuts. Starches
high in oxalates include: amaranth, buckwheat, bran cereal, whole wheat bread and flour, pretzels, and grits.
Popular fruits high in oxalates include: blackberries,
Common High-Oxalate Foods in mg/100g Weight
blueberries, concord grapes, figs, fruit cocktail, kiwis, lemon
Strawberries
peel, lime peel, orange peel, raspberries, canned strawberries,
and tangerines. Vegetables high in oxalates include: beans,
Sweet Potato
beets, carrots, celery, collards, dandelion greens, eggplant,
Chocolate
kale, olives, peppers, potatoes, spinach, summer squash, sweet
Bran Flakes
potato, Swiss chard, and zucchini. Drinks high in oxalates
include: dark beer, black tea, chocolate milk, cocoa, instant
Peanuts
coffee, hot chocolate, and soy drinks (Low Oxalate Diet, 2013).
Almonds
In contrast, low-oxalate foods have less than 2 mg of oxalate
Spinach
per serving and are approved choices for a hyperoxaluria diet.
A few of these foods include: bananas, grapes, beef, poultry,
0
100
200
300
400
500
600
700
800
Oxalate Content (mg/100g weight)
pasta, white rice, cucumber, peas, and milk (Low Oxalate Diet,
Sources used to make graph: (Ritter et al., 2007) and (Weiwen et al., 2007)
2013).
Common Foods
Primary hyperoxaluria is a rare autosomal recessive disorder
(Belostotsky et al., 2010) which involves mutations that lead to enzyme
deficiencies and the overproduction of oxalate. Formation of calcium
oxalate crystals can lead to kidney stones, renal failure, or lifethreatening accumulation of oxalate, known as oxalosis (Messa-Torres
et al., 2013). There are three types of the disorder that can result from
a variety of mutations found on chromosome 2 in type I, on
chromosome 9 in type II, or on chromosome 10 in type III (Hoppe, et
al., 2009).
Medical Treatment
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Treatment of primary hyperoxaluria is dependent on type as well as how much the
disorder has affected the body so far. Hyperhydration is recommended for people with all
forms of primary hyperoxaluria. Restriction of oxalate in the diet is also recommended,
however, one must be careful not to also reduce calcium, which would allow higher oxalate
absorption (Beck et. al., 2013).
Oxalate-degrading organisms have been tested in the past 30 years for the efficacy of treating this disease.
The bacterium Oxalobacter formigenes is an organism that relies solely on oxalate for its energy metabolism. During
pilot trials, orally administered Oxalobacter formingenes decreased oxalate levels in the urine or plasma after four
weeks. Oral administration of Vitamin B6 is a primary hyperoxaluria type I-specific treatment. Pyridoxal-phosphate
(PLP) is one of the six components of Vitamin B6 and is the co-factor of alanine-glyoxylate aminotransferase (AGT). In
type I, the defective enzymes are PLP dependent, meaning that the administration of Vitamin B6 could actually
promote proper folding and function of AGT. Proper function of AGT would allow conversion of glyoxylate into
glycine, rather than into oxalate and glycolate. Other medications such as alkaline citrate and orthophosphate can be
used to treat the effects of primary hyperoxaluria by raising the pH of the urine as well as increasing citrate excretion
through the urine. These factors negatively affect the binding potential of calcium and oxalate, meaning a decreased
amount of calcium oxalate stones would be able to form (Beck et al., 2013).
Dialysis is a treatment typically used in a time period that is as short as possible before transplantation
because dialysis does not successfully lead to oxalate excretion. If the time period is not kept to a minimum, then the
patient may suffer irreversible tissue damage from the oxalate buildup. Transplantation of the liver and/or kidneys is a
treatment option that is taken when no other method has been successful, mainly for people suffering from end-stage
renal disease. Each type of primary hyperoxaluria has different transplant strategies. Transplantation of the liver could
potentially cure the enzyme defect of type I. Kidney transplantation can be combined with liver transplantation or
come afterwards, depending on the state of the kidney and how it is expected to handle the procedure. In type II,
kidney transplants are performed without the combination of a liver transplant because this type is less severe than
type I. Transplantation has not been viewed as necessary in cases of type III (Beck et. al., 2013).