Transcript Synergic effect of the use of anthrax and dental microbial plaque

Synergetic mechanism of the effects of
anthrax and dental microbial plaque
fragments at the design (fashion) of bioweapons and/or cancer treatment
By Antonio Schütz
“Is not necessary to contaminate all an island to proof this mechanism!”
(TN2/2482533)
“The game is equalized”
Synergetic effect of Anthrax toxin and dental microbial plaque
fragments (general mechanism)
•
•
Free
Radicals:
NO∙, O2HOCL∙
RHNCL∙
Mustard gas
Dental plaque
chemoatracts
macrophages Enzime
•
Increases the secretion and synthesis
of free radicals
Improves the formulation of anthrax (aerosol
and particularly paste powder – dry paste)
Increases of the synthesis and
secretion of cytokines production by
NADPH
oxidase
iNOS, MPO
macrophages
“Trojan horse”
Septicemia
•
Increases the effects of LF
•
Increases the effects of EF
•
Increases spores germination to
become vegetative cells within
macrophages
Necrosis
Apoptosis
[cytokines and
growth factors
vase-actives]
Impairs phagocytosis in macrophages
•
Increase of macrophages phagocytose
and lyse endospores
•Decreased lymphocytes function
Monocytes
LPS
Mustard
gas
•
[RANTES]
LPS
Mustard gas
•
Cancer Treatments
–
[IL-8]
•
Chemokynes
(MIP, IP-10, MCP-1)
Hypothension
IL-6
–
–
Oncogenic proteins (Ras) activate
MAPKs
Expression of matrix
metalloproteases
Apoptosis induction
Synergistic effect of Anthrax toxin and dental microbial plaque fragments
Septic
Shock
The anthrax bacteria produce the
anthrax toxin components—
protective antigen, edema factor,
and lethal factor. Protective
antigen (PA) binds to the anthrax
toxin receptor (ATR) on cell
surfaces and forms a proteinreceptor complex that binds lethal
factor (LF) and edema factor (EF).
The toxins enter the cell and
kill it. Most anthrax fatalities are
caused by the irreversible effects
of the anthrax toxins on cells and
tissues.
Apoptosis:
LF (anthrax) and LPS
(dental plaque) induce
apoptosis via
activation TLR
• Acidogenic
dental
bacteria Increase the
effects of PA
Endotoxins of Gran-negatives from dental
microbial plaque increase the synthesis
and secretion of tumour necrosis factor
IL-1, NO, [PGs] as well as their effects
(death, apoptosis, edema) induced by
anthrax toxin.
Endotoxin of
Gram-negatives
of dental plaque
Gramne gative
ce ll
TNF-α, IL-β [PGs],
ON increasing death,
apoptosis and edema]
cytopl.
mem.
pep tid oglycan
oute r mem.
Lipopolysaccharide
(LPS)
Lipid A
Core
polysaccharide
O sidechain
The toxic part
Helps solubilise Lipid A
Somatic antigen
Synergetic activation of NOS
Indomethacin [TNF-α,
[PGs]
IL-1]
Interation with
β-glucan
Immunesuppression
LPS
Anthrax
Dental
plaque
[ET-1]
Death
Septicemia Actives EF
cells
Ulceration of
gastroinstestinal
mucosa
Nephrotoxicity
Increase
the effect
of EF
Edema
Methotrexate [folate]
Mustard gas
Free radicals
Apoptosis
Oxidation of thiois
Lipid peroxidation
Synergistic activation of NOS
increase the production of GMPc
LPS [PGs]
Death
Indomethacin [PGs]
ABthrax specifically recognizes and
neutralizes protective
antigen (PA). By neutralizing PA, ABthrax
effectively
blocks the binding of PA to cell surfaces
and prevents the
anthrax toxins from entering and killing the
cell.
• Evasion of complement and immune response by
structural components ( polysaccharides, sialic acid,
hyaluronic, LPS) and products of synthesis and
secretion of microorganisms of the dental plaque (factor
H, proteases, coagulases, A and M proteins, soluble
antingen), such as stafilococcus, streptococcus able to
block the production of ABthrax antibodies (vaccination)
Chemical components that increase immunesuppressor and/or citotoxic effects
• Chemical substances with structure similar to
Indomethacin and Methotrexate.
• Triazole pesticides
- Inhibition of biosynthesis of steroids
- Low toxicity
- Ineffective against spores
- Peak in 14 days (at plants)
- Variable product formulation: dry and liquid flowables, wettable
powders and emulsifiable concentrates
• Mustard Gas
“The smart bomb”
• Nanocells:
- A new nanoparticle drug-delivery system that makes it
possible to combine drugs (anthrax, dental plaque and
heterocyclic chemical) with different mechanisms of action in
the same particle, and to design their temporal release with
objective of obtaining the synergetic effect.
- Is possible to rise the effects of anthrax toxins with simple
addiction of cytokines (IL-1, TNF…), chemocynes (MIP, IP-10,
MCP-1...) and growth factors ( ERGF…), LPS at concentration
greater than 100 ng/mL, ON, PGs etc…
- In my experience with dental plaque (100 mG/mL) has its
toxicity increased at the short time by indomethacin and
methotrexate, possibly the same occur with chemical with
similar structure.
Conclusion
• As at the construction of a dress, the first phase is the
design. The second is the selection of the color and
texture of the tissue (laboratorial and clinical tests) that
will be used in its construction; the third, the
construction by competent dressmakers. So, the idea of
the design is presented. Now, desire that the colleagues
test it and elaborate the final product. If a dress
originally draw for a fest is modified and used at a
married, is secondary. For the designer what really
import is the design of the idea. Desire this fashion may
be useful for you and particularly to that (TN2/2482533)
incited me the idea of this design. Thank you very much.