Transcript Membrane and Structure and Function (PowerPoint) Madison 2008

Teachable Unit - Membrane
Structure and Function
• Junior level course (cell biology or physiology)
• 3 week unit on membrane structure/function
(including cell signaling/communication)
• Week 1: Membrane components/structure and
transport mechanisms
• Week 2: Properties of membrane-interacting
molecules
This Tidbit
• Late week 2
• Goals
– Reinforce concepts of how molecules interact with
membranes
– Use experimental data to infer how molecules
interact with cells
Learning Outcomes
• Compare and contrast ligand interactions with cells
• Describe different mechanisms by which molecules
interact with cells
• Based on physical properties and concentration
gradients, predict how different molecules interact
with cell membranes
cell
“I have an idea…Let’s start thinking outside of the box….”
What do these
two conditions
have in common?
What do these
two conditions
have in common?
hint
Vitamin deficiency is bad,
so we take vitamins…
but…
After you eat them…
How are vitamins
able to affect cells?
A
B
Outside the cell
cell membrane
Inside the cell
2nd messengers
activated
Changes in cell response
nucleus
What are the similarities and differences
between these two mechanisms?
A
B
Outside the cell
cell membrane
Inside the cell
Changes in cell response
nucleus
Which panel depicts Vitamin D and cells?
A. Left
B. Right
C. No freakin’ idea
2nd messengers
activated
Vitamin D
hint
A
B
Outside the cell
cell membrane
Inside the cell
2nd messengers
activated
Changes in cell response
nucleus
Which panel depicts Vitamin D and cells?
A. Left
B. Right
C. No freakin’ idea
revote
So what about Vitamin C?
Would you predict that Vitamin C
can enter cells…?
Vitamin C
Vitamin C???
n
cultured fibroblasts
Vit C
Vitamin C???
N
Add Vitamin C
Na+
Vitamin C
Vitamin C???
n
cultured fibroblasts
high Na+
w/Na+
low Na+
Add Vitamin C
Outside the cell
Vitamin D
peptides
C
C
C
Vitamin C
cell membrane
C
C
C
Changes in cell response
Inside the cell
nucleus
2nd messengers
activated
Vitamin C - effects on cells and health
•
Collagen metabolism: enhances stability of bones, teeth,
blood vessels, cartilage, tendons and ligaments
(Co-factor in collagen & elastin synthesis)
•
Immune system function: increases activity of antibodies,
neutrophils and phagocytes
•
Nervous system function: neurotransmitter synthesis
•
Anti-cancer: inhibits cell growth
•
Antioxidant function: scavenges free radicals
Homework Assessment:
Using what we learned today…
Construct a Concept Map
Hydrophobic ligand
Hydrophilic ligand
Membrane
Intracellular receptor
Surface receptor
Gene
Signal transduction
Final Summative Assessment
(Possible Exam Questions)
• Given a new molecular structure, students predict (graphically
and in words) interaction with cells. Defend prediction.
• Given a new set of experimental data, students describe
interaction of molecule with cell.
• Given an unknown molecule, students design an experiment to
determine how it interacts with cells, and predict experimental
outcomes.
Diversity of learning/teaching approaches
1.
2.
3.
4.
5.
6.
7.
Brainstorm(A)
Think-pair-share (A/K)
Clicker (N=2: pre- and post-test) (R/K)
Written prediction (R)
Concept map (homework) (V)
Real life examples (K)
Use of images, words, and graphs (V)
V - visual
A - aural
R - read/write
K - kinesthetic
Thanks to….
We would like to thank the Summer Institute
for this opportunity.
We would also like to thank our fearless
leader (MP) for all of her help,
support and patience…and sense
of humor!
Lastly thanks to all of you !
And to our hosts for the hospitality…