Transcript Document

Lecture outline
• The nomenclature of Immunology
• Types of immunity (innate and adaptive;
active and passive; humoral and cellmediated)
• Features of immune responses
• The major cells of the immune system
Why the great interest in Immunology?
• Basic science: understanding a complex
biological system
• Impact on many aspects of human disease
• Immunization is the ONLY approach for
eradicating a disease
• New therapies based on biology
• Potential for major role in emerging
therapies (gene therapy, stem cell therapy)
The functional importance of the immune system
The central questions
• How does the immune system respond to different
infections?
– Different types of microbes are eliminated by
different effector mechanisms, which are
designed to best combat each type of microbe
– Antigens are useful models for studying immune
responses to microbes
The central questions
•
How does the immune system respond to different infections?
– Antigens are useful models for studying immune responses to
microbes
– Different types of microbes are eliminated by different
effector mechanisms, which are designed to best combat each
type of microbe
• Why does the immune system not respond to self
antigens?
• What are the pathogenic mechanisms and clinicopathologic consequences of abnormalities in the
immune system?
Definitions
• Immunity: protection against infections
• Sensitivity: detectable reaction to exposure to
previously encountered substance (antigen)
In clinical practice, it is not possible to measure
protective immunity by challenging with infectious
agents. Immunity is inferred from reactions to
microbial antigens; individuals who show such
reactions are said to be “sensitized”.
Innate and adaptive immunity
Innate immunity: always present (ready to attack); many pathogenic
microbes have evolved to resist innate immunity
Adaptive immunity: stimulated by exposure to microbe; more potent
Properties of adaptive immune responses
The two features that best distinguish adaptive and
innate immunity are specificity and memory
Primary and secondary immune responses illustrate
specificity and memory in adaptive immunity
The concept of clonal selection
Development of specificity and diversity precedes exposure to antigens
Active and passive immunity
Abbas, Lichtman and Pillai. Cellular and Molecular Immunology, 7th edition, 2011
Active immunity: long-lasting protection (memory),
multiple effector mechanisms activated, lag time
Passive immunity: rapid protection, short duration
Cells of the immune system
• Lymphocytes
– Mediators of adaptive immune responses; only
cells with specific receptors for antigens
• Antigen-presenting cells (APCs)
– Specialized to capture, concentrate, and display
antigens for recognition by lymphocytes
– Dendritic cells; macrophages, B cells; follicular
dendritic cells
• Effector cells
– Function to eliminate microbes; include
lymphocytes, granulocytes (neutrophils,
eosinophils), macrophages
Development of B and T lymphocytes
Congenital immunodeficiency diseases are often caused by blocks
at different stages of lymphocyte maturation
Classes of lymphocytes
Abbas, Lichtman and Pillai. Cellular and Molecular Immunology, 7th edition, 2011
c Elsevier
The CD Nomenclature
•
Structurally defined leukocyte surface molecule that
is expressed on cells of a particular lineage
(“differentiation”) and recognized by a group
(“cluster”) of cell-specific antibodies is called a
member of a cluster of differentiation (CD)
•
CD molecules (CD antigens, CD markers) are:
•
•
•
Used to classify leukocytes into functionally distinct
subpopulations, e.g. helper T cells are CD4+CD8-, CTLs
are CD8+CD4Often involved in leukocyte functions
Antibodies against various CD molecules are used to:
•
•
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Identify and isolate leukocyte subpopulations
Study functions of leukocytes
Eliminate particular cell populations
Types of adaptive immunity
Different types
of immune
responses are
mediated by
different classes
of lymphocytes
and defend
against different
types of microbes
Phases of adaptive immune responses
Need for proliferation and differentiation results in delay
(typically 4-7 days) in effective adaptive immunity
Phases of lymphocyte activation
Proliferation keeps pace with replicating microbes (e.g. 1 B cell
--> 4,000 Ab-secreting cells --> ~1012 antibody molecules/hour)
Differentiation: converts lymphocytes into effective defenders
Stages of lymphocyte activation
• Naïve lymphocytes
– Mature lymphocytes that have not previously
encountered antigen; function -- antigen
recognition
– Preferential migration to peripheral lymphoid
organs (lymph nodes), the sites where antigens
are concentrated and immune responses start
Stages of lymphocyte activation
•
Naïve lymphocytes
– Mature lymphocytes that have not previously encountered antigen;
function -- antigen recognition
– Preferential migration to peripheral lymphoid organs (lymph
nodes), the sites where antigens are concentrated and immune
responses start
• Effector lymphocytes
– Activated lymphocytes capable of performing the
functions required to eliminate microbes
(‘effector functions”)
– Effector T lymphocytes: cytokine secretion
(helper cells), killing of infected cells (CTLs)
– B lymphocytes: antibody-secreting cells (e.g.
plasma cells)
Stages of lymphocyte activation
•
•
Naïve lymphocytes
– Mature lymphocytes that have not previously encountered antigen;
function -- antigen recognition
– Preferential migration to peripheral lymphoid organs (lymph nodes), the
sites where antigens are concentrated and immune responses start
Effector lymphocytes
– Activated lymphocytes capable of performing the functions required to
eliminate microbes (‘effector functions”)
– Effector T lymphocytes: cytokine secretion (helper cells), killing of
infected cells (CTLs)
– B lymphocytes: antibody-secreting cells (e.g. plasma cells)
• Memory lymphocytes
– Long-lived, functionally silent cells; mount rapid
responses to antigen challenge (secondary
responses)