Transcript Exercise 9:
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Exercise 9
Introduction to the Eukaryotic Cell
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Announcements
Students
have time to observe Week 8 plates
and finish their LNAs.
Post
Lab 9/Pre Lab 10 are due by the time
your lab meets next.
LNA Eukaryotic
Cell is due next week in lab.
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Goals
To
learn the basic structures associated with
eukaryotic cells.
To
identify the differences between animal and
plant cells.
To
understand the basis of cell culture, and the
changes imposed on cells under culture
conditions.
To
understand the techniques of fluorescence
and electron microscopy and cell culture.
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Animal Cell
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Plant Cell
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The Exercise
Part I: Plant cells: Onion epidermis
Basic cell structure of a plant lacking chloroplasts
Iodine: observe nucleolus
Janus Green: observe mitochondria
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The Exercise
Part II: Animal cells: Human squamous epithelium
Observe differences from the plant cells
Do the mitochondria vary in size from those found in
plant cells?
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The Exercise
Part III: Autofluorescence
View Tradescantia under the fluorescent microscope
Chlorophyll pigments autofluoresce
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The Exercise
Part IV: Anacharis Leaf Cells
View a living plant cell with chloroplasts
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The Exercise
Part V: Electron Micrographs and Cellular Infrastructure
View EM transparencies
Identify organelles
You may want to take notes for future identification (i.e. Exam 2 and
Final)
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Be Able to Identify:
Nucleus
Nuclear envelope
Nuclear pore
Nucleolus
Rough Endoplasmic Reticulum
Ribosome
Mitochondria
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Be Able to Identify:
Golgi complex
top view
section view
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Golgi
top view
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Golgi
section view
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Be Able to
Identify:
Mitochondrion
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Be Able to
Identify:
Nucleus
Vacuole
Chloroplast
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Be Able to
Identify:
Thylakoid
+ Summary of Exercise 9
Part
slide x 3: water / Iodine / Janus Green
size, nucleus, vacuole, cell wall/membrane, chloroplast,
mitochondria (?)
Part
III: Tradescantia
TA
Autofluorescence
Part
II: Cheek cells
slide x 2: water / Janus Green
size, nucleus, cell membrane, mitochondria (?)
Part
I: Onion epidermis
IV: Anacharis
Cytoplasmic streaming