Transcript LTM-1000 Powerpoint Presentation

Conventional Microinjector
 Inject by oil pressure or gas pressure
 If the capillary with less than 0.5 μm tip is used, the capillary
itself will be moved forward due to the pressure.
 With complicated flow path
 Unable to use fine tips under 0.5 μm for injection
 Unable to inject into the cell having rigid cell wall such as plant cells
 Complicated flow path brings on a time-consuming preparation
LTM-1000 : Structure
LTM-1000
Heart-induced expansion of liquid in a sealed capillary
generates high pressure.
Feature
 Extremely-high pressure (approx. 1MPa per 1℃)
makes it possible to use capillaries with the tip
around 0.1-0.5μm without clogging.
 Simple flow path allows to start experiments
right away saving a lot of labor.
Applications
Conventional
Method
Alexa 546-dextran
10K
Alexa 488
LTM-1000
Alexa 546-dextran 10K
50µm
20µm
Injection of antibody into plant cells
50µm
Lucifer Yellow
Injection into the female gametophyte of plant
*Whereas the damage and sample escape appear by the
conventional method, LTM-1000 ensures no damage and
continuous injections without sample escape by using a fine
tip capillary.
Chloroplast
5µm
Injection into chloroplasts of Cyanidioschyzon merolae