Transcript How to give a talk

Oral presentation
Lab meeting
Journal club
Clear
Qual
Thesis
Presentation at meeting
Interesting
Choice of a paper
Goal: 1. On topic
2. Important and interesting to a large audience
Localization AND (Cell [ta] OR Science [ta] OR Nature [ta])
Nature Methods
Introduction
2-3 slides
From general to particular
Introduction-Example 1
Introduction-Example 1
• Transcription
is important in neural stem
Significance?
cells
• SMRT=NcoR2
Question?is a nuclear co-repressor
• Histones get methylated at lysine 27.
What do I need to know?
• There are demethylases that target
these lysines
Scheme/visuals?
• SMRT -/- mice have defects in the
cortex.Punch line?
Neural stem cells
neural stem cells
progenitors
neuronal
glial
Development of the nervous system
Regeneration of adult brain
Transcriptional regulation in
neurogenesis?
SMRT
Program of target genes?
Neuronal fate
Localization of molecules
In situ hybridization of RNA:
Target genes for SMRT
Immunofluorescence:
Role of SMRT on cell fate
(neuronal versus glial markers)
Approaches
1-2 slides
1 scheme is better than 1000 words
Emphasize controls and limitations
Example-Figure1
Title?
Method used?
Legend?
QuickTime™ and a
decompressor
are needed to see this picture.
Are all panels relevant?
Is the Figure relevant?
Conclusion?
SMRT normally prevents
neuronal differentiation
Method: In situ hybridization on brain sections at embryonic
Day 12.5
WT KO WT KO
QuickTime™ and a
decompressor
are needed to see this picture.
Emx2
Mash1
Dlx1
Gad1
Markers of differentiated neurons were increased in SMRT
KO brains
Packaging
Use Arial or Helvetica bold
Black letters
Yellow letters
Use animation *not too much*
Front 24-44
Delivery
Stand up
Look at the audience
Speak loudly + articulate
Display enthusiasm
Practice makes perfect