Transcript High power

Connective tissue (1)
1.Loose connective tissue (No.4, No.1 )
2.Dense connective tissue (No.14)
3.Adipose tissue (No.14/ No.6)
4.Reticular tissue (No.6)
1. General features:
1) small number of cells and large amount of
extracellular matrix (intercellular material).
2) the extracellular matrix is composed of fibers and
an amorphous ground substance.
3) functions: connection, supporting, protecting,
nutrition, defense and repairing.
2. Classification

Connective tissue proper
 Cartilage
 Bone
 blood
The types of CT are determined on the basis of the types of
cell and the characteristics of the extracellular matrix
connective tissue proper
CT in narrow sense means connective tissue proper,
include:
loose CT
adipose tissue
reticular tissue
dense CT
Loose connective tissue
Material: rabbit mesentery
Slide No:4
High power:
1.
Elongated
and
tortuous
collagenous fibers are stained
red and arranged in bundles.
2. The elastic fibers are blue
stained, branched and formed a
woven network.
3. The fibroblast is the cell
most commonly in close to the
collagen fiber. Its basophilic
nucleus is visible.
4. For the macrophage, its
cytoplasm contains some blue
dye substance.
LCT：HE X400
fiber
Low power: fibers are
loosely and irregularly
arranged.
high power:
Elongated and tortuous
collagenous fibers are
stained red and arranged in
bundles.
Elastic fibers are blue
stained,branched and
formed a woven network.
Cell
Macrophage: ingest the
blue dye by phagocytosis
so they can be easily
identified.
Fibroblast: the most
commonly cell in close to
the collagen fiber.only the
basophilic nucles is
visible,but the acidophilic
cytoplasm is not clearly
defined.
Fibroblast
Macrophage
Slide No:1
Method: H.E
lower power:
The lightly stained
submucosa
between
mucosa and smooth
muscle layer.
Small intestine: HE X40
Low power：
1
submucosa
High power:
collagenous fibers are the
predominant component
and they course in
different direction. They
are sectioned in various
planes and cut ends is
seen. stained red and
arranged in bundles.
Fibroblast are the most
numerous cells ,only the
basophilic nucles is
visible,but the
acidophilic cytoplasm is
not clearly defined.
Fibroblast
collagenous fibers
Small intestine: LCT HE X400
Dense connective tissue and adipose tissue
Slide No:14
Material: human skin from
finger
Method: H.E
Naked eye: in the section,
epidermis
is
violent
stained. Under epidermis,
the dermis is pink and
composed
of
dense
connective tissue.
In hypodermis, you can
find
loose
connective
tissue and adipose tissue.
Skin :DCT HE X40
Low power:
In the section ,
epidermis is violent
stained. Under
epidermis,the
dermis is pink and
compose of denes
connective tissue.
epidermis
dermis
high power:
collagenous fibers are the predominant component and they course
in different direction. They are dense ,irregularly arranged and
formed thic bundles. stained red .The numbers of cells are few,
most of which are fibroblasts and fibrocytes.
Dense regularly connective tissue
High power:
Collagenous fibers
are arranged in
compact,parallel
bundles
Tendon cell:
Tendon(longitudinal section)
Reticular tissue
Slide No.5
Material: lymph node of cat
Method: H.E
High power:
Reticular cell: A reticular cell
produces reticular fiber and
surrounds the fibers with its
cytoplasm.
It has many processes. Its nuclei is
lightly stained, but the nucleolus is
clear.
Lymph node: Reticular tissue HE X400
Reticular tissue
Lymph node: Reticular tissue HE × 400
Reticular tissue
①reticular cell ②lymphocyte ③macrophage
Lymph node: Reticular tissue Silver × 400
Adipose tissue
Slide NO: 6
Adipose tissue
Material: human lymph node
Method: H&E
Low power:
Fat cell are present in
large accumulations,
orgnized into adipose
tissue
Lymph node：Adipose tissue HE X40
high power:
Individual Fat cells
show their chracteristic
structure,empty
cells,due to solution of
fat during section
preparation.
The nucleus is pushed
to the periphery of the
cell.
Fibroblast
Macrophage
Mast cells
1.Round and large cell
2.Small dark-stained nucleus
3.Basophilic secreting
granules--metachromatically
Plasma cell
1.Round or ovoid in shape
2.Nucleus with more clock-liked heterochromatin which located
eccentrically
3.Basophilic cytoplasm
Collagenous fibers
collagenous fibril
fibroblast
Mast cell
Plasma cell
macrophagy
The drawing
Collagenous fiber
Macrophage
Elastic fibers
Fibroblast
Loose connective tissue
H&E stain 400×