Transcript Diapositiva 1

Molecular Biology
Medicine Part I
SWITH
Ettore Sansavini Health Science Foundation – ONLUS
Lugo (Ravenna), Italy
Carlo Ventura
Professor of Molecular Biology
University of Bologna, Italy
Now please shift to the cell world
and Regenerative Medicine
Body regeneration
•
We regenerate most of our body approximately
every 3 months.
• So, our body has inherent regeneration potential
and we are naturally targeted to self-healing
• Moreover a population of resident stem cells,
that can drive a regeneration process, is
distributed across the body tissues
Each individual is a small piece of nature
• We live in a multitude of physical signals and
fields: electromagnetic fields, Higgs field,
gravitational field……
• The human body is unceasingly penetrated and
crossed by a number of cosmic particles,
energy waves, electrically charged or not.
Each individual is a small piece of nature
Our body generates a seeming infinity of rhythms
and vibrations expressing the cells’ molecular
dynamics, now recordable at the atomic and
molecular levels.
Different cells may have different languages. If you
record a culture of healthy cells, old cells, cancer
cells you can capture different languages.
Through these reciprocal physical signals, (besides
many biological messages), the cells are able to
organize their decisions (activation of specific
functions, migration, differentiation….. apoptosis)
We live in an active physical environment
In conclusion, the body’s cells both
generate electrical microcurrents and are
sensitive to external physical fields and
acoustic or subsonic vibrations, and can
react with profound functional changes,
including trans-differentiation, until clocking
back to a time zero in which all kind of
decisions are still possible
Novel Findings
Reprogramming human adult stem cells
and human non-stem somatic cells (skin
fibroblasts) into embryonic like stem cells,
capable to differentiate along cardiogenic,
vasculogenic, skeletal myogenic and
neurogenic lineages
Reversing human Stem Cell Aging
Reprogramming Cancer Stem Cells
Ultimate goal
To reprogram the tissue resident
stem cells with no need for stem
cell/tissue transplantation, by a:
non-invasive, inexpensive,
personalized, and easily
deliverable on large-scale bases
strategy.
Cells Have a Vision
Cells are able to detect the orientation of others by signals
that penetrated glass but not a thin metallic film.
These signals are carried by electromagnetic radiation
FROM THE CELL SURFACE TO NUCLEUS,
A WORLD OF COHERENT VIBRATIONS
Labeling with fluorescent tubulin
Released MTs move away from the12
centrosome, out of
the nuclear region and translocate to the cytoplasm
Sahu S et al, 2013
Sahu S et al, 2013
New Concepts on
Biomolecular Recognition
All Life is About Rhythms and
Their Timely Patterning
17
18
Symphony of life, revealed: New imaging technique captures vibrations
of proteins, tiny motions critical to human life
Like the strings on a violin or the pipes of an organ, the proteins in the human
body vibrate in different patterns, scientists have long suspected. Now, a new
study provides what researchers say is the first conclusive evidence that this is
true.
This graphic visualizes the vibrations in lysozyme as it is
excited by terahertz light (depicted by the red wave
arrow). Such vibrations, long thought to exist, have never
before been described in such detail, said lead
researcher Andrea Markelz, ( University of Buffalo).
terahertz near-field microscopy
NATURE COMMUNICATIONS | ARTICLE
Optical measurements of long-range protein vibrations
Gheorghe Acbas, Katherine A. Niessen, Edward H. Snell & A.G. Markelz
Nature Communications 5, Article number: 3076 doi:10.1038/Published 16
January 2014
a singular vibrational entity in a web makes it
exhibiting connectedness
20
Atomic Force Microscopy (AFM)
Single Kinesin Imaging on a Single Microtubule
Schaap IAT et al, 2011
Connectedness is an Evolving Form of
Recognition
Masha Dimitri
Equistasi consists in a rectangular plate measuring
1062060.5 mm and with a weight of 0.17 gr (Figure
1). The device is exclusively composed by
nanotechnology fibers that transform the body
temperature into mechanical vibratory energy
(<0.8N, 9000 Hz) able to generate a variation of
muscle length of max 0.02 mm [36], by far within the
safety limit (0.12 mm) found to be harmful for human
muscles
Interrogation of Nanomechanical Signatures
During Stem Cell Development
(“Nanomechanical characterization of cellular activity - Sonocytology”)
Vibrations arise from the integration of various oscillatory rhythms, from
nanomechanical properties of sub cellular structures up to the cell surface
Sound from single yeast cell
22 °C
26 °C
30 °C
Dead cell
In both the philosophical and visual sense, ‘seeing is believing’ does not
apply to the emerging new discoveries in Science, for which there is
nothing even remotely visible to create proof of existence. On the atomic
and molecular scale, data are recorded by sensing and probing in a very
abstract manner, which requires complex and approximate interpretations.
We are now facing a novel path where visualization and creation of a
narrative becomes necessary to describe what is sensed, not seen.
We have growing needs of separating the “informational content” of Life
from its “material substrate.” “Information” and is deployment into a
multifaceted “knowledge” are the essence of Life.
SWITH (Stem Wave Institute for Tissue
Healing), Ettore Sansavini Health Science
Foundation, Lugo (Ravenna), Italy
Carlo Ventura
Francesca Bianchi
Claudia Cavallini
Elena Olivi
Riccardo Tassinari
SONOLAB, Bologna, Italy
Maurizio Villa
Rinaldi-Fontani Institute, Florence, Italy
Salvatore Rinaldi
Vania Fontani
Alessandro Castagna
Department of Biomedical Sciences
University of Sassari, Italy
Margherita Maioli
Valentina Basoli
Sara Santaniello
Gianfranco Pigliaru
Sara Gualini
Department of Chemistry and Biochemistry
UCLA, LA, USA
James K. Gimzewski
Sarah Cross
Andrew Pelling
Pasteur Institute, Paris
UNESCO, Paris
Luc Montagnier
Cell Transplant Center
Diabetes Research Institute (DRI)
WorldWide DRI Federation
University of Miami, USA
Camillo Ricordi
Carlo Tremolada
INFN, Italy
Emilio del Giudice
Giuseppe Vitiello
Alberto Tedeschi