Transcript Day 4_7 - 335 PDA Detector

ProStar 335 Detector
Petro van Poppel – Field Support Specialist
Tuesday, 12 April 2016
Overview
Introduction to PS 335 Detector
• Dual Array option
• Poly Verify Software
• Flow cell
• Communications
Poly Verify
• Production Tests with 0x0 flow cell
• Wavescan
• (Settings Tab)
• (Input/Output Tab)
• (EEPROM)
• (COMMS Tab)
• (Calibration Tab)
PS 335 Shipping Test
Problems
ProStar 335 Mechanical Optical layout
ProStar 335 Ray Trace Layout
335 Optics
335 Ray Trace
335 Single and Dual Array
Single Array
1024 photodiode sample array
2 photodiode references (250nm and 500 nm)
No extended range spectra
Uses only 9x0 and 4x0 flow cells
Microbore to Analytical applications
Dual Array
1024 photodiode sample and reference array
Extended range spectra (up to 70-100 AU)
Used for analytical to prep apps
Flow Cell Ray Beam
General design of the 9x0 flow cell
General design of the 9x1 flow cell
Flow Cell Specs
Flow Cell
Prep
(9 x 1)
Pressure
rating
1000 psi
Flow Cell
Volume
30 µl
Material (non Ti & PEEK
optical)
Noise
Performance
Super
Prep
(4 x 0.15)
Analytical
(9 x 0)
Micro
(4 x 0 )
1000 psi
1000 psi
1000 psi
145 µl
15 µl
1.5 µl
Ti & PEEK Ti & PEEK
Ti & PEEK
200 ml/min
10-500ml
800 ml/min
0.80-10 L
40 ml/min
0.1 µl-100 ml
1 ml/min
0-20 ml
1x10-4AU
1x10-4AU
5x10-3AU
7.5x10-3AU
Communications
TCP/IP communications for Detector:
•Static or Dynamic
•Networked or standalone
•Firewall
•Access on Network, OS or PC
PS 335 PolyVerify Software
PS 335 PolyDiagnostics
Ctrl-Shift-F10 to enable Diagnostics side
Ctrl-Shift-F9 to hide Diagnostics side
335 PolyVerify Test Certificate
Test Certificate provided by
the factory for each 335
detector
PS 335 PolyVerify Tests
PS 335 Noise Test
PS 335 Wavelength Accuracy test
Run with a 4 nm slit
PS 335 SBW Accuracy Test
Wavelength
Range of 190
to 950 nm
PS 335 Detector Linearity Test
pump head pressure of at least 100 atm using a back
.
pressure restrictor of 40psi
PS 335 Wavescan
PS 335 Wavescan Results
PS 335 Contributions from each Lamp
PS 335 PolyDiagnostics Mode
PS 335 Input/Output Mode
PS 335 Main PWB Power Supply LED’s
A: + 24 V power Supply
B: + 9V and – 9V Power Supply
C: + 5 V Power Supply
C
A
Front of the Unit
B
PS 335 Identifying the Array Type
Knowing the Array type is necessary when running
the Input/Output test diagnostics
PS 335 Dual Array P8 disconnect
P8 connection
PS 335 PolyDiagnostics EEPROM
PS 335 PolyDiagnostics COMMS
PS 335 PolyDiagnostics Calibration Tab
Caffeine Shipping Test
PS 335 Caffeine Shipping Test:
Test made up of 1 components:
• Loop injection using 25 µg/ml caffeine
Test is conducted using Galaxie Software
PS 325/335 Caffeine Loop Injection Test
Test Calculates:
Peak Height
Peak Area
Noise
S/N
Common Problems
•High Noise
•Lamp on, not ready
•SIG_CC_SLIT_PEAK_NOT_FOUND
•SIG_CC_SIGNAL_OVERRANGE_REPREFORM_ LAMP_CAL
•COM_CC_CIRCULAR_BUFFER_OVERRUN _OCCURED
•9555 LMP_CC_D2_FILAMENT_FAILURE
•Foggy Optics
PS 335 Error Messages Table can be found:
• PS 335 Service Manual
Foggy Optics
What is Foggy Optics?
What is Foggy Optics?
Three types of Foggy optics
• White powdery coating
• Rust/brown spot burn
• Speckled mirror
(delamination)
Foggy Optics
What is Foggy Optics?
Diagnosing Foggy Optics
Diagnosing Foggy Optics
Run a “wavescan” test,
When bad
Open the optics and inspect.
Wavescan test results
Intensity for the 254 nm line
Currently is 10 – 50 K counts
False positive
New D2 vs Aged D2 Intensities
Foggy Optics
What is Foggy Optics?
Diagnosing Foggy Optics
Treating Foggy optics
0603 Project (Foggy Optics)
Proposed Fixes and their status:
White powder fogging: due to leaky flow cells and
customer sample depositing on the mirrors
New Flow cells & fittings available for all cells except 4 x 0.15
Brown powder fogging: due to UV attack on some of
the foam parts close to the UV source.
Eliminating as much of the foam as possible and shielding the
rest.
Mirror delamination: due to UV attack on the thin Al
coating of the mirrors
Eliminated by putting a NiCr coating on the mirrors to
strengthen the Al coating.
Opening and cleaning the optics
Cleaning 325 optics
Clean area in circle
Do not adjust!!
Replacing Optics
Wavelength calibration data must be entered into the 335 EEPROM
Replacing Optics
This label is attached to the
top of each optics module,
the shipping box and the
test document
Summary
•Single and Dual Array
•Optical path is open
•Flow cell is dual path
•Diagnostics Tests, like wavescan
•Shipping Test
•Main Problems (Foggy Optics)