Transcript Day 4_6 - 325 UV Detector

ProStar 325 Detector
Petro van Poppel – Field Support Specialist
Sunday, 10 April 2016
Overview
Introduction to PS 325 Detector
• Dual Wavelength Option
• LC Verify Software
• Flow cells
• Communications
LC Verify
• Production Tests with 0x0 flow cell
• Wavescan Test (Mono Options)
• Diagnostics Mode (Main Tab)
• Electronics Test (Input/Output)
• System Details (EEPROM)
• Communications (COMM)
PS 335 Shipping Test
Problems
325 Optics Module Light Path
Vis
D2
325 Optics Ray Beam
Dual Wavelength Option
Dual wavelength is a firmware
change that allows you to view 2
wavelengths “simultaneously”
Galaxie Software
LC Diagnostics Software
325/335 Back Panel
P/N 02-101875-00
Relay Adaptor board
I/O adaptor board
for the sync signals
P/N 02-101865-00
Flow Cell Ray Beam
General design of the 9x0 flow cell
General design of the 9x1 flow cell
Flow Cell Specs
Flow Cell
Prep
(9 x 1)
Pressure
rating
1000 psi
Flow Cell
Volume
30 µl
Material (non Ti & PEEK
optical)
Noise
Performance
Super
Prep
(4 x 0.15)
Analytical
(9 x 0)
Micro
(4 x 0 )
1000 psi
1000 psi
1000 psi
145 µl
15 µl
1.5 µl
Ti & PEEK Ti & PEEK
Ti & PEEK
200 ml/min
10-500ml
800 ml/min
0.80-10 L
40 ml/min
0.1 µl-100 ml
1 ml/min
0-20 ml
1x10-4AU
1x10-4AU
5x10-3AU
7.5x10-3AU
Communications
TCP/IP communications for Detector:
•Static or Dynamic
•Networked or standalone
•Firewall
•Access on Network, OS or PC
LC Verify side
LC Diagnostics Side
Ctrl-Shift-F10 to enable Diagnostics side
Ctrl-Shift-F9 to hide Diagnostics side
PS 325 Test Certificate
Test Certificate provided
by factory
Parameters tested:
1) Noise
2) Wavelength Accuracy
3) Wavelength Reproducibility
4) Spectral Bandwidth
Accuracy
5) AutoZero Precision Test
6) Detector Linearity Test
LC Verify use in Final Testing
PS 325 Noise Test (FT)
PS 325 Wavelength Accuracy Test (FT)
PS 325 Wavelength Repeatability Test
(FT)
PS 325 Spectral Bandwidth Test (FT)
PS 325 Auto Precision Test (FT)
PS 325 Detector Linearity Test (FT)
pump water for at least 10 minutes at a pump head pressure of at least
100 atm using a back pressure restrictor of 40psi.
PS 325 LCVerify Wavescan
PS 325 Wavescan results
PS 325 Contributions from each Lamp
PS 325 LCVerify Diagnostics Mode
PS 325 LCVerify Input/Output Test
PS 325 Main PWB Power Supply LED’s
A: + 24 V power Supply
B: + 9V and – 9V Power Supply
C: + 5 V Power Supply
C
A
Front of the Unit
B
PS 325 LCVerify System Details
PS 325 LCVerify Communications
Caffeine Shipping Test
PS 325 Caffeine Shipping Test:
Test made up of 1 components:
• Loop injection using 25 µg/ml caffeine
Test is conducted using Galaxie Software
PS 325 Lamp Intensity Test
Test 1: Lamp Intensities
PS 325/335 Caffeine Loop Injection Test
Test Calculates:
Peak Height
Peak Area
Noise
S/N
Common Problems
•High Noise
•Lamp on, not ready
•SIG_CC_ZERO_ORDER_PEAK_FIND_FAILED
•SIG_CC_SIGNAL_OVERRANGE_REPREFORM_ LAMP_CAL
•COM_CC_CIRCULAR_BUFFER_OVERRUN _OCCURED
•9555 LMP_CC_D2_FILAMENT_FAILURE
•PROSTAR 325 DRIVER: Collection not started - Acquisition time too short for
the method.
•Foggy Optics
PS 325 Error Messages Table can be found:
• PS 325 Service Manual
• Dealernet
Foggy Optics
What is Foggy Optics?
What is Foggy Optics?
Three types of Foggy optics
• White powdery coating
• Rust/brown spot burn
• Speckled mirror
(delamination)
Foggy Optics
What is Foggy Optics?
Diagnosing Foggy Optics
Diagnosing Foggy Optics
Run a “wavescan” test,
When bad
Open the optics and inspect.
Wavescan test results
Intensity for the 254 nm line
Currently is 10 – 50 K counts
False positive
New D2 vs Aged D2 Intensities
Foggy Optics
What is Foggy Optics?
Diagnosing Foggy Optics
Treating Foggy optics
0603 Project (Foggy Optics)
Proposed Fixes and their status:
White powder fogging: due to leaky flow cells and
customer sample depositing on the mirrors
New Flow cells & fittings available for all cells except 4 x 0.15
Brown powder fogging: due to UV attack on some of
the foam parts close to the UV source.
Eliminating as much of the foam as possible and shielding the
rest.
Mirror delamination: due to UV attack on the thin Al
coating of the mirrors
Eliminated by putting a NiCr coating on the mirrors to
strengthen the Al coating.
Opening and cleaning the optics
Cleaning 325 optics
Clean area in circle
Do not adjust!!
Replacing Optics
Wavelength calibration data must be entered into the 335 EEPROM
Summary
•Single and Dual Wavelength, UV and UV/vis
•Optical path is open
•Flow cell is dual path
•Diagnostics Tests, like wavescan
•Shipping Test
•Main Problems (Foggy Optics)