Transcript Document

Nervous Tissue
刘尚明 武玉玲
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Multipolar neuron:
Cross section of spinal cord of dog.
This specimen is elliptic in shape. In the
center of it there is a H shaped structure,
the gray matter, stained deeply.
1. Gray matter 2. White matter
Low mag: Spinal cord
Low power:
The multipolar
neurons can be found
in the anterior horns of
the gray matter. The
cell bodies can be
round or ovoid. The
other smaller and round
nucleus are the nucleus
of glial cell.
1. Multipolar neuron
2. The nucleus of glial
cell
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High power:
The nucleus is
large, round, palely
stained, centrally
located and with a
prominent
nucleolus. The
cytoplasm contains
many granular
areas, the Nissl
bodies, stained in
dark blue color.
1. Nucleus
2. Nucleolus
3. Nissl body
4. Axon hillock
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3
4
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High power: Multipolar neuron
1. Soma
2. Neurite
3. The nucleus of glial cell
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High power: Multipolar neuron
1. the nucleus
2. dendrite
3. axon
Section of myelinated nerve fiber (H&E stain)
This is a section of dog sciatic nerve.
It is stained in red color. The elongated
structure is the longitudinal section and the
round, the cross section.
Low power: This is
the longitudinal
section, the whole
nerve is
encapsulated in a
dense connective
tissue covering, the
epineurium. Many
parallel cord
shaped structures
can be seen, they
are the myelinated
nerve fibers and
stained in purplered color.
1. Nerve fiber
2. Epineurium
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Cross section of
nerve fiber:
1. Epineurium
2. Perineurium
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2
High power:
longitudinal section
1.Node of Ranvier
2.axon: appears as a thread
shaped, centrally located
structure, stained in purplered color.
3.Myelin sheath: envelopes
the axon, stained in light red
color or presenting a foamy
appearance.
4. neurolemma : the thin
thread beside the myelin
sheath. And you can see the
nucleus of Shchwann’s cell is
elongated and located
approximately halfway
between 2 adjacent nodes
and appear to lie in
indentation of the myelin.
: nucleus of Shchwann’s
cell
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2
4
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Cross section:
Each nerve fiber appears
as a round structure. The
central dot shaped
structure is the axon,
which is surrounded by
myelin sheath, stained in
very light color or being
foamy. The nucleus of
Schwann’s cell presents
a round shape.
1. Axon
2. Myelin sheath
3. Neurilemma
4. Perineurium
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4
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Cross section:
1. axon
2. Myelin sheath
3. Neurilemma
4. Perineurium
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Meissner corpuscles and Pacinian corpuscles:
Section of human skin. The epidermis is
stained deeply, which is made up of keratinized
stratified squamous epithelium. The dermis is
stained lightly, which is composed of connective
tissue.
Low power:
Meissner corpuscles
are located in the
dermal papillae, they
are elongated
structures composed
of a thick connective
capsule. They
usually oval shaped.
1. Meissner
corpuscle
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High power:
1. Meissner
corpuscle: it is
enveloped by
connective tissue,
with a few layers of
pinacocyte in it,
which constitute
the connective
tissue cradle of the
tactile corpuscle.
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Vater-Pacini
corpuscles:
can be found in
the deep layer of
the dermis. You
can see an oval
corpuscle with
many layers of
concentric
arranged
pinacocytes.
Motor end plate:
Spread preparation
of skeletal muscle of
cat, gold chloride
stain.
Low power: The
muscle fibers are
stained in brown
color, but the nerve
ending, in black.
The neural element
is claw shaped
structure, attached
to the surface of
muscle fiber.
1. Muscle fiber
2. Motor end plate
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2
High power:
The claw shaped
ending of the
motor end plate is
usually expanded
as a button.
1. Motor end plate
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Cerebra:
This section is part of brain
hemisphere.
Low power: the cerebra is
covered by connective tissue.
the leptomeninges ( ) . The
hyperchromatic tissue is the
cortex, and the light stained
tissue in the deep part is the
medulla. Cortex is composed
of all kinds of nerve cells, and
can divide into 6 layers. To
distinguish the 6 layers is not
easy in this section, but you
can distinguish the shape of
the pyramidal cell, with a tip
facing the surface of the
cortex.
1. Cortex
2. Pyramidal cell
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2
High power:
The nucleus of
the nerve cell is
round and light
stained. The
nucleus of glial cell
is small and dark
stained.
1.Nucleus of
nerve cell.
2.Nucleus of glial
cell
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Cerebellum:
Distinguish the
cortex and the medulla
of the cerebellum.
There are three layers
in the cortex, the
molecular layer, the
Purkinje cell layer and
the granular layer. The
cells in the molecular
layer and the granular
layer is difficult to
distinguish. But the
Prrkinje cell is big and
easy to recognise.
1.Molecular layer
2.Purkinje cell layer
3.Granular layer
4.Medulla
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High power:
To observe the
construction
features of the
Purkinje cell.
1.Purkinje cell
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Cerebellum:
Stained with
silver nitrate in
order to observe
the form of the
Purkinje cells.
Please pay
attention to the
branch of the
dendrite.
1.Purkinje cell
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Spinal ganglion:
Low power: The
ganglion cells are
separated by
parallel nerve fiber,
and form a cells
group.
1.Myelinated nerve
fiber
2.Ganglion cell
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High power：
Spinal ganglion
Cells are inequality
of size, and have
one big and round
nucleus, which is
light stained, with a
evident nucleolus.
There is a layer of
flat cells around
the ganglion cell,
the satellite cell,
the nucleus of
which is small and
dark stained.
1.Ganglion cell
2.Satellite cell
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Sympathetic
ganglion:
Low power:
The size of
the neuron in the
sympathetic
ganglion is small
and similar.
1. Ganglion cell
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High power:
The ganglion
cells are
scattered, the
nucleus of
which is often
at one side.
You can not
see the neurite
in the section.
1.Ganglion cell
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