Transcript gametogenesis thru gastrulation

Seminiferous Tubules (human)
Ovary (cat)
Ovum (squirrel)
Primordial Follicle (human)
Secondary Follicle (human)
Addition of Yolk to Oocyte by Follicle Cells
Maturing Secondary Follicle (monkey)
Secondary Ooocyte in Antral Follicle (human)
Oogenesis – formation of polar bodies
(rat, human, and mouse, respectively)
(Click on the link to run the animation)
Meiosis
http://biology-web.nmsu.edu/houde/MEIOSIS.mov
(Close the movie window to return to this presentation)
Frog (Xenopus)
Oogenesis
http://biology-web.nmsu.edu/houde/frogoogenesis.mov
Fertilization - Acrosome Reaction and Cortical Reaction
Sea Urchin
rhodamine phalloidin staining
of fertilization cone
(explosive actin polymerization)
http://biology-web.nmsu.edu/houde/Rhphalloidinstainingfertilizationcone.mov
Inositol triphosphate (IP3) is a second messenger released from phosphatidyl inositol
bisphosphate (PIP) by phospholipase C, and binds to and activates a calcium channel in
the endoplasmic reticulum, releasing a wave of Ca++ that spreads across the ovum.
Calcium ion stimlates the release of cortical granules to the perivitelline space, which
widens as a slow block to polyspermy and deactivates bindin receptors.
Sea Urchin
Cortical Reaction
phase contrast left
intracellular Ca++ right
http://biology-web.nmsu.edu/houde/mk0780641001.mov
Sea Urchin
fusion of egg and
sperm pronuclei
http://biology-web.nmsu.edu/houde/Fusioneggspermpronuclei.mov
Ascidean Cortical Rotation
frog (Xenopus)
cortical rotation
(follows gravitational
rotation)
http://biology-web.nmsu.edu/houde/corticalrotation.mov
frog (Xenopus)
gravitational rotation,
Cleavage
http://biology-web.nmsu.edu/houde/fert2.mov
Branchiostoma Cleavage and Blastulation
Xenopus Blastulation
frog (Xenopus)
Blastulation
http://biology-web.nmsu.edu/houde/xenopus embryo.mov
Branchiostoma Gastrulation
Xenopus Zygote Cytoplasmic Fate Map
Xenopus median section blastulation – bottle cells
Xenopus Vegetal and Median Gastrulation
Frog (Xenopus)
Gastrulation,
formation of yolk plug,
Neurulation
http://biology-web.nmsu.edu/houde/RKellergastrulation.mov
Xenopus Median and Parasagittal Gastrulation
Frog (Xenopus)
Gastrulation – median
optical section
http://biology-web.nmsu.edu/houde/froggastmedianopticalsection.mov
Keller sandwich
Cutout (frog)
http://biology-web.nmsu.edu/houde/sandwich_cutout.mov
Keller sandwich explant
(chordaneural elongation)
http://biology-web.nmsu.edu/houde/Kellersandwichdorsallipexplant.mov
Branchiostoma Neurulation
Xenopus Neurulation