Transcript Slide 1

Using and Understanding
95% Confidence Intervals
in BIOL 1011
# of bubbles produced
(your bench)
mean # of bubbles produced
(n= 6 )
100 cm
(light off)
100cm
(light on)
50cm
(light on)
25cm
(light on)
12 cm
(light on)
0
3
15
38
49
0.25
4.35
18.65
36.20
51.15
# of bubbles produced
(your bench)
mean # of bubbles produced
(n= 6 )
100 cm
(light off)
100cm
(light on)
50cm
(light on)
25cm
(light on)
12 cm
(light on)
0
3
15
38
49
0.25
4.35
18.65
36.20
51.15
are they different?
100 cm
(light off)
100cm
(light on)
50cm
(light on)
25cm
(light on)
12 cm
(light on)
0
3
15
38
49
0.25
4.35
18.65
36.20
51.15
# of bubbles produced
(your bench)
mean # of bubbles produced
(n= 6 )
are they different?
mean # of bubbles produced
(n= 6 )
mean # of bubbles produced
(n= 6 )
100 cm
(light off)
100cm
(light on)
50cm
(light on)
25cm
(light on)
12 cm
(light on)
0
6.75
20.00
41.65
62.85
100 cm
(light off)
100cm
(light on)
50cm
(light on)
25cm
(light on)
12 cm
(light on)
1.1
2.25
14.85
22.45
38.35
100 cm
(light off)
100cm
(light on)
50cm
(light on)
25cm
(light on)
12 cm
(light on)
0
3
15
38
49
0.25
4.35
18.65
36.20
51.15
# of bubbles produced
(your bench)
mean # of bubbles produced
(n= 6 )
mean # of bubbles produced
(n= 6 )
mean # of bubbles produced
(n= 6 )
100 cm
(light off)
100cm
(light on)
50cm
(light on)
25cm
(light on)
12 cm
(light on)
0
6.75
20.00
41.65
62.85
100 cm
(light off)
100cm
(light on)
50cm
(light on)
25cm
(light on)
12 cm
(light on)
1.1
2.25
14.85
22.45
38.35
sample
means for
Bacopa at
50 cm with
100W light
sample means vary around the population mean
14.85
18.65
20.00
sample means vary around the population mean
14.85
18.65
20.00
standard error tells us how much a sample mean tends to vary from the population mean
sample means vary around the population mean
14.85
18.65
20.00
standard error tells us how much a sample mean tends to vary from the population mean
standard error = standard deviation / √ n
sample means vary around the population mean
95% of sample means
fall within 2 standard errors
of the population mean
2SE
14.85
18.65
2SE
20.00
standard error tells us how much a sample mean tends to vary from the population mean
standard error = standard deviation / √ n
sample means vary around the population mean
so, an interval of
sample mean ± 2 standard errors
is 95% likely to contain the
population mean:
it’s a 95% confidence interval
95% of sample means
fall within 2 standard errors
of the population mean
2SE
14.85
18.65
2SE
20.00
standard error tells us how much a sample mean tends to vary from the population mean
standard error = standard deviation / √ n
Using 95% confidence intervals to compare two means
Calculate standard error (SE) for each mean using standard deviation and n
Construct 95% confidence interval for each mean: sample mean ± 2SE
Compare the two intervals: do they overlap?
NO
the means are likely to be
significantly different
YES
the means are not likely to be
significantly different
Location
Mean Age
(years)
Standard
Deviation
n
West End
3.50
0.9
556
East End
3.60
0.7
442
Does mean age differ between west and east?
Location
Mean Age
(years)
Standard
Deviation
n
West End
3.50
0.9
556
East End
3.60
0.7
442
Does mean age differ between west and east?
Null hypothesis: There is no difference between mean age at west and east ends.
Location
Mean Age
(years)
Standard
Deviation
n
West End
3.50
0.9
556
East End
3.60
0.7
442
Does mean age differ between west and east?
Null hypothesis: There is no difference between mean age at west and east ends.
1. Find standard error for each.
2. Construct 95% confidence intervals for each.
3. Construct graph to visually compare confidence intervals. Do they overlap?
Maternal Age at Birth of First
Offspring
3.7
3.65
sample means
3.6
3.55
3.5
3.45
3.4
error bars
(mean ± 2SE)
3.35
3.3
West End
East End
Location on Island
3. Construct graph to visually compare confidence intervals. Do they overlap?
Maternal Age at Birth of First
Offspring
3.7
3.65
3.6
3.55
3.5
3.45
3.4
3.35
3.3
West End
East End
Location on Island
The intervals overlap, so we can say that the means are not likely to be different.
We will not reject the null hypothesis that the means are not different.