Transcript Integrating Cells into Tissues

Ｉｎｔｅｇｒａｔｉｎｇ
Ｃｅｌｌｓ into Tissues
Sho Momota
CAM(Cell-adhesion molecules)
ECM(Extra cellular matrix)
ECM
Proteoglycans
Collagen fibers
Multiadhesive matrix proteins
CAM
Cadherins
Ig (immunoglobulin) super family
Selectins
Mucins
integrins
Cadherins
Ca
cell
membrane
N-teminus
Extra cellular
region
50-60% of the sequence is identical
among different cadherins.
C-teminus
Cytoplasm
Types of cadherin
More than 40 different cadherins are known
E-cadherin :
P-cadherin :
N-cadherin:
Preimplantation embryos,
non-neural epethelial tissue
Trophblast
nervous system, lens, cardiac and
skeletal muscle
Character of cadherin
Homophilic (like bind like)
E-cadherins bind to E-cadherins
P-cadherins bind to P-cadherins
E-cadherins usually not bind to P-cadherins
N-CAMs
Group of Ca-independent cell-adhesion proteins
Ig super family of CAMs
Primarily Homophilic interaction
Encoded by a single gene.
Selectin
Extravasation of
leukocytes
require the
successive formation
and breakage of
cell-cell contacts
between leukocytes
in the blood and
endothelial cells
lining the vessels
Blood
Sialyl
Lewis-x
antigen
Sugar binding
lectin domain
P-selectin
Endothelial cell
Integrin
Heterodimers of  and  subunits
Exhibit relatively low affinities for the ligands
IIb3 integrin
platelet
Collagen or
thrombin
Accelerates a
formation of clot
Conformational change of IIb3 integrin
platelet
Fibrinogen
Activated platelet
Ｄｅａｄｈｅｓｉｏｎ factors
Promote cell migration and can remodel the
cell surface
Disintegrins
RGD sequence
ECM
De-adhesion factors
CELL
CAM
Disintegrins
ECM
Question?