Transcript Slide () - Anesthesiology - American Society of Anesthesiologists

From: Syntaxin1A-mediated Resistance and Hypersensitivity to Isoflurane in Drosophila melanogaster
Anesthesiology. 2015;122(5):1060-1074. doi:10.1097/ALN.0000000000000629
Figure Legend:
Isoflurane decreases transmitter release. (A) A schematic of the Drosophila larval neuromuscular junction (NMJ) recording
preparation is shown. Ground for stimulation wire (gray) and recording ground wire shielded with nail polish (black) plus perfusion
set up (blue) with the vacuum (orange) is shown. The recording electrode (red) is impaled into muscle 6 of segment 3 of dissected
third-instar larvae. A stimulating electrode (green) encases the nerve, which innervates the muscle. Dashed lines represent a more
detailed schematic of muscle 6 synaptic boutons (purple arrowheads) which release glutamate onto the muscle. Not all muscles or
nerves
are shown3/24/2017
for simplicity, and the
brain is
in this schematic
for illustration purposes
only
(brain is removed when
Date of download:
Copyright
© shown
2017 American
Society of Anesthesiologists.
All rights
reserved.
recording). (B) i: Time course of NMJ experiment, showing duration of the recording (in min) with red arrow denoting when isoflurane