Transcript 10 tcp/rer/3402/sucec

FAO TCP/RER/3402
“Assistance to Western Balkan Countries for Improving Compliance to International Standards
on Aquatic Animal Health“
Outputs of the National Workshop in Croatia,
Ivica Sučec, DVM
Regional Workshop 6: Project Output Dissemination and Terminal Workshop
7-9 April 2015, Hotel Hollywood, Sarajevo, Bosnia and Herzegovina
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FAO TCP/RER/3402
“Assistance to Western Balkan Countries for Improving Compliance to
International Standards on Aquatic Animal Health“
FAO TCP/RER/3402
“Assistance to Western Balkan Countries for Improving
Compliance to International Standards on Aquatic
Animal Health”
National workshop, Zagreb, February 19, 2015
Regional Workshop 6: Project Output Dissemination and Terminal Workshop
7-9 April 2015, Hotel Hollywood, Sarajevo, Bosnia and Herzegovina
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FAO TCP/RER/3402
“Assistance to Western Balkan Countries for Improving Compliance to
International Standards on Aquatic Animal Health“
Under the project “Assistance to Western Balkan Countries for Improving Compliance to
International Standards on Aquatic Animal Health”, it is also envisaged that each
participating country organize national workshop.
Therefore, on February 19, national workshop was held in Zagreb, in order to:
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Present activities during FAO project in past two years
Introduce farmers on new approach for disease control in Croatia
Present results from antimicrobial resistance baseline study conducted in 2013/2014
Present future activities of farmers as regards health status of freshwater farms
One day workshop was held in Coratian Economic Chamber, with presence of 31
participants in total.
Regional Workshop 6: Project Output Dissemination and Terminal Workshop
7-9 April 2015, Hotel Hollywood, Sarajevo, Bosnia and Herzegovina
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FAO TCP/RER/3402
“Assistance to Western Balkan Countries for Improving Compliance to
International Standards on Aquatic Animal Health“
Assistance to Western Balkan Countries for Improving
Compliance to International Standards on Aquatic
Animal Health, Sanin Tanković, DVM
The purpose of the presentation was to explain background of TCP, background to TCP/RER/3206, TCP Regional
Facility TCP/RER/3206, and finally TCP/RER/3402 Regional TCP
Regional Workshop 6: Project Output Dissemination and Terminal Workshop
7-9 April 2015, Hotel Hollywood, Sarajevo, Bosnia and Herzegovina
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FAO TCP/RER/3402
“Assistance to Western Balkan Countries for Improving Compliance to
International Standards on Aquatic Animal Health“
Antimicrobial resistance baseline study results in 2013/2014, Dražen Oraić, DVM
In introductory part of presentation, antibiotics, their role and mechanism how they work
when applied were described. Furthermore, WHO findings on possible resistance were
described, as main problem if antibiotics will be used in future, with no strategy and control
of famers and competent authority. Each year, around 25000 deaths are noted, in relation to
ATB resistance. Aquaculture producers use ATBs mainly in order to increase production by
preventing diseases. There are three typs of resistance: clinical, fenotyp and genetic. In
Croatia, in 2013. baseline study on ATB resistance was launched, and continued in 2014 as
ATB resistance programme. Following bacteria were examined for resistance: Yersinia ruckeri,
Flavobacterium psychrophila, Vibrio anguillarum, Tenacibaculum maritimum. Samples were
tested on five antimicrobials: Oksitetraciklin, Trimetoprim, Florfenikol, Flumekvine,
Eritromicin. As result, resistance is detect for OTC, Trimetoprim and Flumekvine in Vibrio
anguillarum and Tenacibaculum maritimum and for OTC and Trimetoprim in Yersinia ruckeri,
Flavobacterium psychrophila. Results impose action plan for several sectors, human,
veterinarian, etc..Goal is to lower the impact of ATB resistance in people by prudent use of
ATB, by producing manuals for farmers, and continuos monitoring.
Regional Workshop 6: Project Output Dissemination and Terminal Workshop
7-9 April 2015, Hotel Hollywood, Sarajevo, Bosnia and Herzegovina
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FAO TCP/RER/3402
“Assistance to Western Balkan Countries for Improving Compliance to
International Standards on Aquatic Animal Health“
Overview of the most important diseases in Croatian freshwater aquaculture during last
five years, Snježana Zrnčić, DVM
Presentation entitled “Overview of the most important diseases in Croatian
freshwater aquaculture during last five years” was intended to inform participant
about occurrence of the most important listed and economically important diseases
and about the diseases which may endangered national aquaculture industry.
Although Croatia is implementing surveillance programme with regard to viral listed
diseases according to national legislation based on the Council Directive 2006/88/EC
there were two single outbreaks of both listed viral diseases on two different
Croatian farms. In July 2013., high mortalities in rainbow fry were noticed. Affected
fish showed exophtalmia, pale gills, distended abdominal wall with haemorrhages on
the skin, in the eye, on the liver, pyloric caeca, swimbladder, intestine, in dorsal
musculature and enlarged spleen.
Regional Workshop 6: Project Output Dissemination and Terminal Workshop
7-9 April 2015, Hotel Hollywood, Sarajevo, Bosnia and Herzegovina
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FAO TCP/RER/3402
“Assistance to Western Balkan Countries for Improving Compliance to
International Standards on Aquatic Animal Health“
VHS virus was isolated on cell cultures, identified by ELISA and confirmed by RT-PCR and by sequencing it was
found that virus belongs to genotype Ia, subtype 2, grouped into subclade Pol II. In December 2013., samples
from newly established rainbow trout were submitted to NRL as a part of routine monitoring programme.
Affected fish showed exophthalmia, pale gills, hemorrhages on the skin, fat tissue, pyloric caeca, peritoneum
and intestines, liver and in dorsal musculature. Cell culture examination resulted by virus isolation and
identification was performed by ELISA, confirmed by RT-PCR and by sequencing results we concluded that IHNV
isolate belongs to M group. In both cases we could not find out the source of infection. Later on viral diseases
were spread to another farms so totally six farms are considered infected. Participants were informed about
main symptoms diagnostic procedure for detection of Infectious pancreatic necrosis (IPN), Rainbow trout fry
syndrome (RTFS), bacterial gill disease and Red mark syndrome in rainbow trout cultivation. They were also
informed about diseases that my threaten carp production like listed disease Koi herpes virus disease (KHV)
and other diseases like Spring viraemia of carp (SVC), Swim bladder inflammation (SBI) and carp
erythrodermatitis CE).
Regional Workshop 6: Project Output Dissemination and Terminal Workshop
7-9 April 2015, Hotel Hollywood, Sarajevo, Bosnia and Herzegovina
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FAO TCP/RER/3402
“Assistance to Western Balkan Countries for Improving Compliance to
International Standards on Aquatic Animal Health“
Aquaculture strategy in Croatia, Emin Teskeredžić, DVM
In this presentation, overview of main obstacles and future plans for Croatian
Aquaculture were presented, beginning from drought in 2010., breakdown of
supports from government, predators, high fees for water management and most
recently, outbreaks of viral haemorrhagic septicaemia and infectious haematopoietic
necrosis on trout farms. As conclusion, on behalf of trout producers association, it is
agreed that eradication of trout diseases (VHS, IHN, IPN) is of utmost importance.
Producers gave positive examples in other countries with eradication programmes,
which were successful, and virus was eradicated (USA, Denmark).
Regional Workshop 6: Project Output Dissemination and Terminal Workshop
7-9 April 2015, Hotel Hollywood, Sarajevo, Bosnia and Herzegovina
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FAO TCP/RER/3402
“Assistance to Western Balkan Countries for Improving Compliance to
International Standards on Aquatic Animal Health“
Aquatic animal heatlh surveillance programmes in 2015, Ivica Sučec, DVM
In this presentation, participants were informed about aquatic animal surveillance
conducted on trout and carp farms registered in Croatia. Results were shown from
2009, and consisted surveillance data for VHS/IHN, IPN and Koi herpes virus disease in
period from 2009 till 2014. In 2009. Surveillance program was launched in order to
collect data from all trout farms in Croatia, in order to achieve disease free status for
VHS/IHN. Results of surveillance were satisfactory till 2013. Since then, six outbreaks
of VHS/IHN were noted, and epidemiological situation in Croatia is no longer
favorable. As regards KHV, surveillance programme was also conducted on all farms
with susceptible species, and in five years of targeted surveillance with negative
results, conditions for disease free country were achieved. Due to fact that VHS/IHN
virus is present in 6 farms in Croatia, sampling and investigation of wild population
was conducted in December 2014. Results showed that in all susceptible species in
open waters, virus was not found. Furthermore, presence of Onchorhyncus mykiss
was much less than expected, and brown trout is known to be less susceptible to viral
diseases such as VHS. Sampling of wild species was conducted on three rivers
(Kupčina, Dretulja i Gacka).
Regional Workshop 6: Project Output Dissemination and Terminal Workshop
7-9 April 2015, Hotel Hollywood, Sarajevo, Bosnia and Herzegovina
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FAO TCP/RER/3402
“Assistance to Western Balkan Countries for Improving Compliance to
International Standards on Aquatic Animal Health“
Furthermore, farmers were introduced to plan for surveillance of aquatic diseases in 2015. It is envisaged that
farmers themselves will have much bigger role in decision making for their surveillance programmes in 2015.
VHS/IHN surveillance is envisaged for all trout farms which are already under surveillance, and for those that
are infected, eradication programme is also envisaged. Furthermore, programmes for achieving additional
guarantees, in order to be more competitive in EU market, and no to have any obstacles for free trade of live
fish. One of such proposals is program for Spring viremia of carp. Forms for submission of application for this
surveillance program, and for declarations of disease free status will be drafted and delivered to farmers.
After filling in the form, application will be evaluated, and declaration and/or draft surveillance/eradication will
be presented and sent to EC for approval.
Regional Workshop 6: Project Output Dissemination and Terminal Workshop
7-9 April 2015, Hotel Hollywood, Sarajevo, Bosnia and Herzegovina
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