Transcript WV Department of Agriculture

Animal Health Division
Brenda Keavey
Aquatic Health Certifications
West Virginia Department of Agriculture’s Laboratory in Charleston
History
• first fish bacterial cultures performed - 1990’s
• WV Dept. of Education samples
• Few requests from producers
(approximately 2 requests annually)
http://www.picsearch.com/pictures/animals/fish/fish%20to-z/trout.html
West Virginia Department of Agriculture’s Laboratory in Charleston
Progression of Laboratory Testing for Fish
2006
2007
Sampling training at Micro Technologies Inc.
Diagnostic testing training at Micro Technologies Inc.
 Sampling procedures training
 Testing areas included bacterial, viral, molecular and parasitic
West Virginia Department of Agriculture’s Laboratory in Charleston
Progression of Laboratory Testing for Fish
2008
Obtained Viral Hemorrhagic Septicemia Virus (VHSV) permit for
positive cultures.
Prepared for DNR testing – USDA grant for surveillance of VHSV.
Performed viral cell culture transfers and testing
samples for approximately 1 year.
2009 Suspended cell culture testing due to lack of
samples and direction of lab’s role in aquaculture.
2010 Cell culture testing reinstated at the end of the year due to the
formation of the Aquaculture Board.
West Virginia Department of Agriculture’s Laboratory in Charleston
Current Laboratory Testing Goals for 2011- Bacterial Cultures
Bacterial cultures for a number of organisms
Aeromonas species
Streptococcus species
Carnobacterium piscicola
Vibrio species
Edwardsiella species
Yersinia ruckeri
Flavobacterium species
West Virginia Department of Agriculture’s Laboratory in Charleston
Current Laboratory Testing Goals for 2011 - Training
Rob Nottingham, Laboratory Supervisor
Animal Health Division
Microbiologist
Regulatory and Environmental Affairs Division
West Virginia Department of Agriculture’s Laboratory in Charleston
Current Laboratory Testing Goals for 2011- Certifications
Become certified by USDA to perform cell culture for
Viral Hemorrhagic Septicemia Virus (VHSV)
Infectious Hematopoeitic Necrosis Virus
(IHNV)
Cytopathic effect in FHM cell line 4 days after inoculation
Courtesy of AFS Bluebook
West Virginia Department of Agriculture’s Laboratory in Charleston
Laboratory Testing Goals for 2011 – 2012
Bacterial Diseases of Fish
Renibacterium salmoninarum
(Bacterial Kidney Disease)
Cultural Techniques
Fluorescent Antibody Test (FAT)
Polymerase Chain Reaction (PCR)
Enzymed-Linked Immunosorbent
Assay (ELISA)
Courtesy of 2010 edition of the American Fisheries Society, Fish Health Section Blue Book
West Virginia Department of Agriculture’s Laboratory in Charleston
Laboratory Testing Goals for 2011 – 2012
Viral Diseases of Fish
Infectious Pancreatic Necrosis Virus (IPN)
Cell Culture
Fluorescent Antibody Test (FAT)
Polymerase Chain Reaction (PCR)
Birnavirus: The Queen's University of Belfast,
kindly provided by Prof Stewart McNulty
West Virginia Department of Agriculture’s Laboratory in Charleston
Laboratory Testing Goals for 2011 – 2012
Parasitic Diseases of Fish
Tetracapsuloides bryosalmonae
Proliferative Kidney Disease (PKD)
Presumptive diagnosis - staining
confirmation histopathology
Courtesy of 2010 edition of the American Fisheries Society, Fish Health Section Blue Book
West Virginia Department of Agriculture’s Laboratory in Charleston
Laboratory Testing Goals for 2011 – 2012
Parasitic Diseases of Fish
Ceratomyxa shasta
Myxobolus cerebralis
(Whirling Disease)
ANY QUESTIONS?