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A new method to measure 10B uptake in
lung adenocarcinoma in hospital BNCT
E. Capelli, P. Chiari
F. Basilico, P. Mauri
A. Zanini
D. Bolognini, E.M. Donegani,
A. Mattera, M. Prest, S. Hasan
Page  1
P. Cappelletti, M. Frigerio,
S. Gelosa, A. F. Monti
G. Giannini, E. Vallazza
F. Borasio
Outline
BNCT
The INFN PhoNeS project
An innovative approach to Boron Imaging
Neutron Autoradiography
Measurements and results
Conclusion
Page  2
Boron Neutron Capture Therapy
10B
(n,α,γ) 7Li
binary technique
BNCT could become useful for
highly-fatal tumours:
•Extended (e.g. liver, lung)
•Radioresistant (melanoma)
•Localized near vital organs
(glioblastoma)
Page  3
high LET particles
Difficulties for BNCT applications
Low toxicity
High specificity
High intensity
thermal-epithermal
neutron beam
(5·108 n/cm2 s1)
Non-persistence in the
bloodstream
< 10 keV energy range
Gamma contamination
< 2 ·10-13 Gy cm2 / n
BPA
Page  4
BSH
Neutron Autoradiography
 Nuclear reactor
 Solid state track detector (CR 39)
 Sometimes cryogenic temperature during
the irradiation
 Optic microscope for identification and count
 Hospital-based neutron source
 Real time detector
Page  5
PhoNeS (PhotoNeutronSource)
Neutron flux in the treatment cavity: Φ = (1.3 ± 0.1) x 107 n cm-2 s-1
γ-contamination: reduced of 97%
Page  6
Real-time Neutron Autoradiography
0.5 cm CERROBEND
photonconversion
6 cm PMMA
thermalization
IRON BOX
•Samples
Neutron flux:
Φ = (1.5 ± 0.1) x 105 n cm-2 s-1
Page  7
•Detector
The imaging detector
Silicon Dimensions
(9.5 x 9.5) cm2
Hamamatsu Microstrip Silicon Detector
768 strips
Readout pitch
242 μm
Read-out by
3 self-triggering TAA1 ASICs
Counts vs position
Page  8
The Data Acquisition System
• UNDEPLETED silicon detector
to detect α-particles from boronated samples down to 25 ng of 10B
• INTER-BUNCH acquisition
18 MeV electrons
Bunches of 5 μs
Frequency 100 - 300 Hz
Page  9
The samples
SOLUTION HOMOGENEITY
to prevent crystallization
and self-absorption of
the α-particles by the
sample itself
SPECIMEN SUPPORT
-plastic support (liquid solution)
evaporation
-glass slide (blood samples)
coagulation process
KNOWN VOLUME
Drops of 5 μl
Page  10
Calibrating with BSH
Integrated Spot Content (AU) vs
Concentration (ppm)
500 MU/min –18 MV photons
Varian CLINAC 2100 C/D, Sant’Anna Hospital (Como)
Page  11
The first ever kinetic curves
Blood and urine of a patient administered BSH, taken at different times with respect to the administration.
BLOOD
URINE
rescaled at the max value obtained by Si-autoradiography
Page  12
Special thanks to Dr.Sauerwein
Ex-vivo lung perfusion
in collaboration with “S.Luigi” Hospital in Orbassano (TO), Italy
Human lung affected by
adenocarcinoma:
• therapetic resection
• perfusion of blood and BPA
Blood samples taken at
different times:
• to estimate 10B uptake
Page  13
Kinetic curves
• Blood samples doped with BPA
• Comparison between real-time
autoradiography to MS analysis
• The absorption takes place
only in the first 90mins
Page  14
CR 39
10B
concentration in tumour and healthy tissues of resected lobe
High-resolution images
to reproduce the features
of the tissues
Unhealthy tissue
CT / CH ~ 1.5 - 2
Healthy tissue
Page  15
Special thanks to Dr. Giannini
Conclusions
 Neutron Autoradiography in a hospital environment
successfully performed - Linac
 Development of an hospital-base neutron source
to perform Boron Imaging with a 1D spatial resolution
 Real-time detector to perform pharmacokinetic studies
results during the irradiation
 Blood samples from a perfused lung affected by adenocarcinoma
kinetic curves - 10BPA down to concentrations of 5ppm
 Evaluation of 10B concentration in tumour and healthy tissues
Si-autoradiography – CR 39
 Looking forward to make BNCT possible in every radiotherapic unit
Page  16
A new method to measure 10B uptake in
lung adenocarcinoma in hospital BNCT
A. Zanini
E. Capelli, P. Chiari
F. Basilico, P. Mauri
Thank you!
G. Giannini, E. Vallazza
P. Cappelletti, M. Frigerio,
S. Gelosa, A. F. Monti
Page  17
D. Bolognini, E.M. Donegani,
A. Mattera, M. Prest, S. Hasan
F. Borasio