Transcript Method of Isolating Shikimic Acid From a Plant

U.S. Patent and Trade Office, Provisional patent application no.
60/841,643 filed August 30, 2006,
“Method of Isolating Shikimic Acid from a Plant”
Inventor: K.A. Anderson.
• Shikimic acid is present is most plants but in low
concentrations
• Shikimic acid is naturally concentrated in chinese star
anise seeds
• Star anise is used in the pharmaceutical industry as a
base material for production of Tamiflu (oseltamivir).
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U.S. Patent and Trade Office, Provisional patent application no.
60/841,643 filed August 30, 2006,
“Method of Isolating Shikimic Acid from a Plant”
• Star anise seed as a base material for production of Tamiflu
– About 15 to 40 grams of star anise is needed to produce 10 tamiflu vaccines
– Only 1 crop per year can be grown in China only
– 10 step manufacturing process
– Low isolation and yield of shikimic acid from star anise seed blamed for 2005
shortage of tamiflu
– 90% of harvest is used by Roche to make Tamiflu
– According to Roche major bottle neck to Tamilfu production is availability of
shikimic acid
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“Method of Isolating Shikimic Acid from a Plant
-Bio-enhanced Synthesis for Preparation of Shikimic Acid”
• Round-up interupts shikimic
acid pathway in plants
• Results in build-up (high
concentration) of shikimic acid
in plants
• Very fast response
• Very large increase
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“Bio-enhanced Synthesis for Preparation of Shikimic Acid”
• Apply Round-up modest
amount so plant continues to
grow further simplifing harvest
• Multiple crops could be grown
per year if needed
• Simple extraction of plant
material
• High yield of shikimic acid
from plant
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Chemical Marker for Glyphosate Applications
-Shikimic acid in Wheat
4000
Shikimic Acid ug/g
3000
2000
1000
0
0
5
10
15
Days from Initial Treatment
Wheat Control
Glyphosate Low Application Rate
Glyphosate High Application Rate
20
25
“Method of Isolating Shikimic Acid From a Plant”
• 60 grams of alfafa would
produce enough shikimic
acid for 10 Tamiflu vacines
– Per acre seeds versus alfafa
shikimic acid production
potential is large
– Analytical clean easier
• Water extraction of plant
• Prepatory chromatography
clean-up/isolation
Science Supporting Society
U.S. Patent and Trade Office, Provisional patent application no.
60/841,643 filed August 30, 2006,
“Method of Isolating Shikimic Acid from a Plant”
Inventor: K.A. Anderson.
• Outlook ??
– US approved purchase of 25 million doses of Tamiflu
– US wants to stock pile >30 million doses
– Requires 8.4 million grams of shikimic acid
• In 2005 10 g of shikimic acid cost $5
• 8.4 million grams = >$4,000,000.00
– SE Asian estimates: 2.9 BILLION vacines are needed
– Other vacines for bird flu
– Other sources of shikimic acid
– Other methods to produce Tamilfu without using shikimc acid
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