Transcript Micro-Spherical Heart Pump Powered by Cardiomyocytes

MICRO-SPHERICAL HEART PUMP
POWERED BY CARDIOMYOCYTES
Yo Tanaka1, Kae Sato1, Tatsuya Shimizu2, Masayuki
Yamato2, Teruo Okano2, Takehiko Kitamori1
1The
University of Tokyo, Bunkyo, Tokyo, 113-8656, Japan
2Tokyo Women’s Medical University, Shinjuku, Tokyo, 162-8666, Japan
Ref: μTAS2006
報告者: 9633584 黃紫郁
Outline

Introduction
 Other
Micro-Pump
 This Group’s Micro-Pump




Materials and Methods
Results and Discussion
Conclusions
References
Introduction: Other Micro-Pump
Electric Power
Electric Powerless
(Zengerle, et al. ,1995 IEEE)
(J.H. Tsai, L. Lin, 2001 IEEE)
Jungyul Park, et al. , 2007 Lab Chip
(C. Cabuz, S.T. Lu , 2001 IEEE)
Introduction: This Group’s Micro-Pump
Medium
Lab Chip, 2006, 6, 362–368
Lab Chip, 2007, 7, 207–212
Materials and Methods


A hole (600 μm in diameter) in
the center of sugar ball and
edible silver was detached.
Teflon capillary (200 μm in
inside and 400 μm in outside
diameters) was threaded
through the hole and molten
glucose by using a hotplate at
150 ℃ was applied around
the hole.
Materials and Methods


About 1 mL of PDMS
prepolymer solidified at 100
℃ for 1 hour above a
hotplate under rotation (22
rpm).
Drawing and insertion of
capillaries, and the two
capillaries were attached to
sphere using epoxy glue.
Materials and Methods



One capillary was connected
to a syringe pump and
dissolving sugar ball in water.
A hollow sphere (about 5 mm in
diameter, 250 μm in thickness)
with connected capillaries was
fabricated.
The PDMS sphere was sterilized
and immersed for 1 h in 50 μg
mL-1 fibronectin solution in PBS
at 37 ℃ to promote
cardiomyocyte attachment.
Results and Discussion


A cultured cardiomyocyte
sheet produced periodic
contractile-expansion
motion of the PDMS micro
spherical heart.
Spherical polystyrene
tracking particles (1 μm
diameter) were dispersed
in cell culture medium
within the capillaries.
Results and Discussion
Before transplantation
After transplantation
Conclusions



New fabrication methods to create a novel micro spherical
heart-like pump prototype.
Regular fluid motion in a capillary connected to the hollow
pumping sphere, with the device working continuously over 5
days.
This device is a fully integrated, wireless mechanochemical
converter, driven with only chemical energy input from culture
milieu.
Conclusions


1.
2.
External control of both fluid motion and mechanical
performance of the bio-actuator is possible using culture
temperature.
Possible applications:
as an electric powerless bio-actuator to drive fluids in
implanted micro-chemical or biochemical medical implant
devices.
as a component of a cardiovascular circulatory system micromodel to study mechanisms of circulatory physiology,
pathology, and developmental biology.
References
1.
2.
3.
4.
Y. Tanaka, K. Morishima, T. Shimizu, A. Kikuchi, M. Yamato, T. Okano, T.
Kitamori, Lab Chip, 6, 362-368, (2006).
Y. Tanaka, K. Sato, T. Shimizu, M. Yamato, T. Okano and T. Kitamori, Lab
Chip, 2007, 7, 207–212.
Y. Tanaka, K. Sato, T. Shimizu, M. Yamato, T. Okano, Ichiro Manabe, Ryozo
Nagai and T. Kitamori, Lab Chip, 2008, 8, 58-61.
Jungyul Park, Il Chaek Kim, Jeongeun Baek, Misun Cha, Jinseok Kim, Sukho
Park, Junghoon Lee and Byungkyu Kime, Lab Chip, 2007, 7, 1367–1370