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Lab-on-a-Chip
The Ideal Technology for Bio-chemical
Analysis
Xingyu Jiang Ph.D.
[email protected]
National Center for Nanoscience and Technology, China
What is “Lab-on-a-chip”?
A lab-on-a-chip (LOC) is a device that integrates one or
several laboratory functions on a single chip of only millimeters
to a few square centimeters in size.
What can “Lab-on-a-chip” do?
Biochemical assays: real-time PCR,
immunoassay, dielectrophoresis for
detecting cancer cells and bacteria, etc.
Chemical application: separating
molecules from mixtures, chemical
reactors, chemical detections. etc.
Biological application: cell coculture,
biosensor, drug screening, single-cell
analysis, etc.
Automation for Lab-on-a-chip
Development of Microfluidic Large Scale Integration
with thousands of mechanical valves
Parallel Assays Made Easy!
Microfluidic Diagnostic Methods
Portable Point-of-Care (POC) Medical Diagnostic Systems
The chips should be inexpensive, accurate, reliable and
suited to the medical conditions in developing countries.
How to Prepare a Microfluidic Chip?
L-Edit, AutoCAD
Print
Photolithography
or etching
Soft embossing
The Method for Preparing a Chip in Our Lab:
Soft embossing by polymer (PDMS etc.)
A method for analyzing HIV antibodies in
microfluidic channels
PDMS:Polydimethylsiloxane
PC: Polycarbonates
Improved efficiency in assays
Antigens
Advantages:
Fast (20 min.)
High throughput
Low cost
Antibodies
Immunoassay results
on PC membrane
Micro Western blot for detecting multiple
proteins on one chip
Detection of multi-proteins one time
1-3µL Antibody solutions are required
Loop-mediated isothermal amplification (LAMP) integrated
on microfluidic chips for detection of pathogen
Jiang, et al, Anal. Chem. 2010,82, 3002
Cells on a Chip
Microfluidic technology in cell coculture
We developed a method for patterning multiple types of
adherent cells on the same substrate.
Naked-Eye Based
Assays
Nanotechnology in chemical analysis