Transcript Simple stains

Simple stains
By
monifah
Staining
properties
Stains may be:
Simple stains: using one stain only such as methylene .1
blue, carbol fuchsin.
Microscopic examination2
4/12/2017
Dr. Emad AbdElhameed Morad
Preparation of
smears
Put a drop of water on the middle of the slide
using the inoculating needle.
With the sterile needle, collect bacteria from
agar surface by touching the bacterial growth.
Rub the tip of the needle on the glass slide in the
drop of water in a circular motion till you get
homogenous smear.
Allow the smear to dry.
Microscopic examination3
4/12/2017
Dr. Emad AbdElhameed Morad
Fixation of
smears
o Pass the slide with the smear side uppermost
over the flame for 2-3 times.
o Do not overheat the smear. The slide should only
be warm when you touch it with hands.
o Passage of the smear through heat has two
benefits:
1. Fixation of the smear to the slide.
2. Killing of the bacteria in the smear so it becomes non
infectious.
Microscopic examination4
4/12/2017
Dr. Emad AbdElhameed Morad
Staining of
smears
o Cover the smear with methylene blue or carbol fuchsin or crystal
violet for 1 minute.
o Pour it off then wash with water.
o Wash with water.
o Place the film at angle to air dry or blot dry with filter paper.
Microscopic examination5
4/12/2017
Dr. Emad AbdElhameed Morad
Examination of
smears
o
o
o
o
o
Rack the condenser high and open the iris diaphragm.
Place a drop of immersion oil on the smear.
Put the slide with the smear side up on the stage.
Use the oil immersion lens.
Lower the oil lens till the lens contacts the oil and
almost touches the smear.
o Look through the eye piece of the microscope.
o Focus on the object using the coarse adjustment screw
then the fine one.
Microscopic examination6
4/12/2017
Dr. Emad AbdElhameed Morad
Interpretation of
smears
Comment on the bacterial morphology as regards: o
Size
Staining
Shape
Arrangement
Microscopic examination7
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Dr. Emad AbdElhameed Morad
bacteria
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