Transcript CC-124 wild type mt

“Creepy Crawlers”
Chlamydomonas Cultures
Jessica Murrell and Jessica Thompkins
Hillside High School
Durham, NC
Science House
North Carolina State University
Abstract
• Chlamydomonas is a green algae, that has two
flagella and has chloroplast for photosynthesis.
The purpose of this experiment was to see if the
Chlamydomonas algae can be trained to live
from their normal conditions. In our
experiment we found that no significant change
was shown so we were not able to tell if the
algae were adapting to the new environments.
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Introduction
 Chlamydomonas
-Chlamydomonas reinhardtii is a unicellular green alga.
Chlamydomonas swims with two flagella, and has chloroplasts for
photosynthesis.
 10 microns in diameter
Flagella are 10 microns long.
 grows quickly
 forms colonies on plates
 two mating types
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Types of Chlamydomonas
CC-124 wild type mtCC-125 wild type mt
CC-1036 pf18 mt
CC-1101 eyl mt
CC-2679 mbol mt
CC-2894 ptx1
CC-2990 neg1
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Statement of the Problem
 To train the bacteria Chlamydomonas algae to
adapt and behave in normal circumstances when
placed in a light or dark atmosphere.
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Hypothesis
 Chlamydomonas algae has the ability to be
trained to adapt to light and dark
atmospheres in a period of weeks
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Materials
• CC-124 wild type mt• CC-125 wild type mt+
• CC-1036 pf18
mt+
• CC-1101 eyl mt• CC-2679 mbol mt+
• CC-2894 ptx1
• CC-2990 neg1
 5 ml salts solution
 5 ml Phosphate
Solution
 1 ml trace elements
 Microscope
 Slides
 Bulb lights
 Batteries
 Stop Watch
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Methods
 Make Solution for cultures
 Observe to see whether
with salt, phosphate and trace
culture will swim toward
element solution.
light or darkness on the
 Construct a slide with light
light bulb slide.
bulbs and batteries to observe  Record this procedure
the cultures under speculation
everyday for one week.
 Measure the time it takes for
each Chlamydomonas culture  Measure to see how many
time the culture moved
to return to the bottom of test
toward the dark and how
tube after has been shook.
many times it moved
 Put every other tube into light
towards light
or darkness for at least 12
hours switching them on a
daily bases for accurate
results
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Data for Shaken Method
CC-124 wild type mtCC-125 wild type mt+
CC-1036 pf18 mt+
CC-1101 eyl mtCC-2679 mbol mt+
CC-2894 ptx1
CC-2990 neg1
 8 min
 5min
 10min-do not go down
 10min-do not go down
 10min-do not go down
 10min-do not go down
 7 min
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Data for Light and Dark Method
Types
CC-124
CC-125
CC1036
CC1101
CC2679
CC2894
CC2990
Day 1
dark
light
dark
dark
light
dark
neutral
Day 2
dark
light
dark
dark
light
light
light
Day 3
light
light
light
dark
light
dark
light
Day 4
dark
light
light
dark
light
dark
light
Day 5
dark
light
light
dark
light
light
light
Day 6
dark
light
dark
dark
neutral
light
light
Day 7
light
light
light
dark
light
light
Light
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Results
 Judging by the methods of training used, there was no real
conclusive statement that could be made on the bacteria in
training. Our results varied form day to day and were not
persistent at all.
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Discussion
• Further Implications
-Can alter the researches results by using
using a better supply of sunlight and or different
cultures of bacteria
Problems-Some of the algae died and perhaps
contaminated the algae alive causing varied results
and an inconclusive resolution.
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Conclusion
 The algae used in the
• Inconclusive
experiment was not
trained and did not
adapt to its new
environment due to
dead and or
contaminated algae or
the unnatural sun light
used to train the
bacteria.
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Acknowledgements
 Our Family and Friends
 Ms. Beth Snoke and Dr. David Haas
 Ms. Shawna Young
 North Carolina State University
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