Transcript Lab Safety and Sterile

LAB SAFETY
Apparel
• Shoes
• Lab coats
• Safety glasses
Note!
No eating, drinking, or smoking
in the lab at any time.
Spills
• Wash
• Notify
• Receive instructions
Accidents
• Be careful!
• Pay attention
• Report any injuries
Other Matters
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Broken glass
Other waste
Pipetting
Volatile chemicals
Dirty labware
Labeling
General cleanliness
ASEPTIC
TECHNIQUE
I. Contaminants
A. Bacteria, fungi, and insects
B. Initial contaminants
C. Latent or persistent contamination
D. Introduced contamination
E. Detection of contaminants
THE TRANSFER HOOD
• The hood should remain on
–Necessary items present,
unnecessary things removed
• Check the bottom of the hood for
blockage
• Wash hands
THE TRANSFER HOOD
• Spray or wipe the inside of
the transfer hood
• Wipe hands and lower arms
with 70% EtOH
• Remove dangling sleeves
and jewelry
Sterilization and use of
supplies and equipment
• Know which of your implements, flasks,
etc. are sterile and which are not
• Monitor autoclave time
• Sterilized items should be used within a
short time
• Items that come packaged sterile should
be examined carefully for damage before
use
Sterilizing tools, vessels etc.
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Autoclaving
Ethylene oxide gas
UV radiation
Dry heat
Microwave
Working in the transfer hood:
• Work well back in the transfer hood
• Make sure that materials in use are to
the side of your work area, so that
airflow from the hood is not blocked
• Instruments (scalpels, forceps) can be
sterilized by flaming or immersion in hot
beads
Working in the transfer hood:
• Plant material should be placed on a
sterile surface when manipulating it in
the hood
• Remove items from the hood as soon
as they are no longer needed
• When transferring plant cultures, do
contaminated cultures last.
Working in the transfer hood:
• When finished in the hood, clean up
after yourself.
• Be sure when you are finished that you
turn off the gas to the burner
• It is pointless to practice good sterile
technique in a dirty lab
Surface-sterilizing plant material
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Care of stock plants
Alcohol
Bleach
Calcium hypochlorite
Mercuric chloride
Hydrogen peroxide
Rinsing
In the media
• Use of antibiotics and fungicides
• Plant Preservative Mixture
• Rinsing