Transcript Fab

Immunology #10-11
Antibodies and Antigens
II
Ab structure
Fab: Ag binding
Fab: Ag binding
Fc: Effector function
What is the ‘hinge’ good for?
Fc
Ag determinant
Lost by denaturation
Anti-phospho-tyrosine Abs
Gained by denaturation
Proteolysis dependent
Methods that
use Abs
ELISA
IP
Chr.
WB
FACS
Staining
Quansys
Methods that
use Abs
ELISA
IP
Chr.
WB
FACS
Staining
Methods that
use Abs
ELISA
IP
Chr.
WB
FACS
Staining
Methods that
use Abs
ELISA
IP
Chr.
WB
FACS
Staining
Methods that
use Abs
ELISA
IP
Chr.
WB
FACS
Staining
Cases
inhibits release of
name is ____Tetanospasmin
and I’m a 7-year-old male.
I injured
glycine
and GABA
from inhibitory
leg while playing in the yard. I had no significant
interneurons.
My
my
past medical history and all of my vaccinations are upto-date. Several days after the injury, anaerobic cultures
of a wound aspirate were found to grow Clostridium
tetani. Despite the bacterial presence, I never
developed tetanus! Which of the following allowed this?
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A. Sensitized CD8 lymphocytes capable of killing the bacteria
B. Activated macrophages capable of killing the bacteria
C. Neutrophil infiltration of the wound with lysosomal enzyme release
D. CirculatingVaccine:
antibodies
that activate complement
formaldehydeinactivated
tetanus
toxin
E. Circulating
antibodies
that
neutralize bacterial products
Immediate: Tetanus immune globulin
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My name is ____ and I’m a 23-year-old
immigrant that gave birth to a term newborn at
home. My husband cut the umbilical cord with a
kitchen knife. One week later, my baby
developed rigidity and spasms. His condition
could most likely have been prevented by which
of the following?
A. Infant vaccination at the time of birth
B. Maternal vaccination during pregnancy
C. Multivitamins during pregnancy
D. Peripartum antibiotics to the mother
E. Proper infant nutrition after birth
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1
The protein structure shown on
the slide below is most likely to
be found in which of the
Fetal blood contains maternal lgG that was able to
following?
cross the placenta. At approximately 20 weeks of
gestation the fetus does have circulating B
lymphocytes and some amount of fetal 1gM is
detectable in the blood.
Interesting: the secretory piece is
bloodsynthesized by epithelial cells and prevents
IgA degradation by secretions
A. Maternal
B. Fetal blood
C. Fetal thymus
D. Maternal bone marrow
E. Colostrum …. Also mucus, tears and saliva.
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Two patients are vaccinated against
poliomyelitis. One patient receives the killed
vaccine and the other patient receives the live
attenuated vaccine. One month after the
vaccination, the levels of which of the following
poliovirus antibodies will differ most between
these patients?
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A. Serum IgM
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B. Serum lgG
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C. Serum IgA
D. Cerebrospinal fluid IgG
E. Duodenal IgA
My name is ____ and I’m a 23-year-old primigravida
that came for a routine prenatal check-up at 28 weeks
gestation. I have no significant past medical history.
After reviewing the results of my laboratory evaluations,
my physician rapidly gave me an injection of Rh
immunoglobulin.
Rh immunoglobulin belongs to which of the following
immunoglobulin classes?
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A. lgA
B. lgG
C. lgM
D. lgD
E. lgE
erythroblastosis fetalis
maternal lgG anti-Rh
antibodies crossing the
placenta, entering the
fetal circulation, and
causing massive
hemolysis of fetal
erythrocytes.
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Treatment: Anti-Rh Ab
(Rh0GAM) consists of
lgG anti-Rh antibodies
and is routinely
administered to Rhnegative females.
My name is ___ and I’m a gram-negative
bacteria isolated from the blood of a 22-yearold Caucasian female. I produce a protease
that splits the IgA molecule at the hinge region.
Which of the following is the most likely
biological significance of this enzyme?
This protease is produced by
A. It impairs opsonization and phagocytosis
Neisseria gonorrhoeae and
B. It promotes
intracellular
survival
Neisseria
meningitidis
C. It impairs complement-mediated cell lysis
Point of interest: Secretory IgA does not act
facilitates mucosal penetration
asD.
anItopsonin.
E. It impairs secondary immune response generation
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My name is ____ and I’m a 7-year-old male
is brought to the emergency room with Henoch Schonlein2
purpura
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colicky abdominal pain and arthralgias. My
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stool is positive for occult blood and
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urinalysis reveals mild proteinuria. I
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subsequently developed palpable lesions
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over my lower extremities, which are most
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likely caused by:
A. Disseminated bacterial infection
IgA production and immune
History: In his 1801 book, On Cutaneous
complex formation. Deposition
B. Circulating
immune
Diseases, Heberden
described a 5-year-old
boycomplexes
of these IgA immune
with "bloody points" over the skin of his legs,
C. IgE-dependent basophil degranulation
complexes on blood vessel
abdominal pain, bloody stools and urine, and
walls and within the renal
painful subcutaneous
edema.
D. Antibody-dependent
cellular
cytotoxicity
mesangium activates
Johan Schönlein
complement and leads to
Eduard Henoch
E. Delayed hypersensitivity
reaction
inflammation.
‘Leukocytoclastic vasculitis’.
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My name is _____ and I’m an apparently healthy
12-year-old Caucasian male; I came to the clinic
after recurrent exposure to a bacterial antigens
that resulted in a rapid increase in serum IgG
level. My GP explained: “Some immunoglobulin
molecules are attached to the surface of
macrophages, neutrophils and B lymphocytes.” So
I asked her: “Which of the following is the cell
attachment site for the immunoglobulin molecule
shown on the slide below?”
A
B
C
D
E
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My name is ______ and I’m a 34-year-old
Caucasian female whose lgG autoantibodies attack
an antigen and cause cell lysis after binding the C1
complement component.
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Which of the following is the complement binding
site for the immunoglobulin molecule shown on the
slide below?
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A
B
C
D
E
Next lesson:
Another ‘so far’ lesson including:
(A)Small ‘keep-up-with-the-course’ quiz
(B) Extended in-depth topic:
Is [juvenile] type 1 [autoimmune]
diabetes a T-cell or B-cell mediated
disease?