Transcript 10 Mitochondria

The Mitochondria!
Review of Membranes…
D
G
A
F
C
E
B
A
B
C
G
D
E
F
MITOCHONDRIA
Which of the following can get
into the outer membrane?
Outer membrane with porins
Outer compartment (cytosol)
Matrix
Inner membrane with Cristae
Enzymes!
Hexokinase (adds P to ADP) and Dehydrogenases (catalyze redox
reactions)
Mitochondria
• Double membrane
– outer membrane
– inner membrane
• highly folded cristae
• enzymes & transport
proteins
– intermembrane space
• fluid-filled space between
membranes
Mitochondria — Structure
• Double membrane energy harvesting organelle
– smooth outer membrane
– highly folded inner membrane
• cristae
– intermembrane space
• fluid-filled space between membranes
– matrix
• inner fluid-filled space
– DNA, ribosomes
– enzymes
• free in matrix &
intermembrane
membrane-boundspace
cristae
inner
membrane
outer
membrane
matrix
What cells would have a
lot of mitochondria?
mitochondrial
DNA
Mitochondria – Function
Dividing mitochondria
Who else divides like that?
Membrane-bound proteins
Enzymes & permeases
bacteria!
What does this tell us about the
evolution of eukaryotes?
Endosymbiosis!
Advantage of highly folded inner
membrane?
More surface area for membranebound enzymes & permeases
The Real Deal
The Real Deal
Enzymes on the Outside
• Hexokinases- adds P onto ADP
• Dehydrogenases- catalyze REDOX
– Transfers electrons (H) to an acceptor NAD or FAD
• FAD and NAD are electron carriers