Transcript DanielaGoltsman-MicrobialDiversity_session1

Exploring Microbiology
By: Edima Akpan
Marianelly Lopez
Kelechi Okereke
About Microbiology
• Microbiology is simply the study of microscopic
organisms that are in every place thought
imaginable
• Crucial and important to gain information and
understand microbial diversity in order to find the
origins of how viruses and molecular diseases
develop
• To be a microbiologist, one must be curious about
small microscopic life, study microorganisms from
various areas, and be rigorous in the study of
microbiology
Procedures and Techniques
• Inoculation
• Culturing
• Isolation
• FISH (Fluorescence in Situ
Hybridization)
Results From Culturing in Petri Dishes
After (Incubation #2 After
Isolating One Colony)
Before (Incubation #1)
(collected from
the Memorial
Pond)
After
Before
•Organisms were pinkish
•Small circles, dots
•Big worm-like shape
•The head of the “worm” in a
pentagonal shape with the white
pinch at the top
•Tiny bubbles around the
structure
Results continued…
Before (Incubation #1)
After (Incubation #2 After
Isolating One Colony)
(collected from the
bottom of a shoe)
• White cells stick together
• One spot of separation in
colonies
• Single colony in the middle
of agar dish was the same
color
More results…
• Rough edges with wrinkled white
pinches in the center
• Off-white color and cells clumped
together
• Wrinkled pinch has a rotational
symmetry design
Before (Incubation #1)
After (Incubation #2 After Isolating
One Colony)
(collected from refrigerator)
Microscopy Results
• All samples had unviersal bacteria
• There were no eukaryotes in any of
the samples
• All samples contained Gamaproteobacteria
• Marianelly and Edima’s samples
contained Archaea and
Actinobacteria as well
FISH Pictures
• Marianelly: Red,
universal
Bacteria; yellow
(orange) and
green, Gama
Proteobacteria;
blue, Archaea
and
Actinobacteria.
FISH Pictures Continued…
• Edima: Red,
universal
Bacteria; yellow,
Gama
Proteobacteria;
blue, Archaea
and
Actinobacteria.
FISH Pictures Continued…
• Kelechi: Red,
universal
Bacteria; yellow
(orange) and
green, Gama
Proteobacteria.*
Conclusions
• We were capable of discovering microbial
variety in our chosen environmental
samples.
• In addition, we could grow microorganisms
in the lab.
• Lastly, we were successful in identifying
organisms within the Bacteria and Archaea
domains.
• Overall, our experiment was a success.
Our Experiences From This
Project
Extras: Tree of Life
Constructed with 16S rRNA