Transcript Winograwdsky Column

Winogradsky Column
• Bacteria exhibit wide range of diversity
• Prokaryotic organisms keep our world turning
by recycling elements needed for life
• Winogradsky and Beijerinck studied
relationship between different species of
microbes in mixed communities
• 250g mud
• 125g mud, 5g calcium carbonate, 5g calcium
sulfate, 10g shredded paper (cellulose)
• Mixed them together filled to 3cm
• Added rest of the mud to fill up the column
(up to 5cm from the top)
• Made in October 2011
Rhodospirllum
• Grey – Clostridium cellulose degrading
bacteria grows when oxygen is depleted
• Ferments glucose makes acetic acid succinic
acid
• Desulfovibrio uses fermentation products in
anaerobic respiration uses sulfate as the
electron accepter makes hydrogen sulfide
• Makes the mud black
• H2S helps the anaerobic photosynthetic
bacteria to grow
• Green sulfur bacteria Chlorobium
• Purple sulfur bacteria Chromatium
• Use H2S for photosynthesis
• Sulfur goes back to Desulfovibrio
• Most of the column red/rust due purple
nonsulfur bacteria, Rhodospirillum
• Get energy from light, use organic acids made
by Clostridium for carbon. Cannot tolerate
high conc. Of H2S
• Cyanobacteria are in the upper zone