Transcript final ppt

What Are You Drinking,
TEA?
Tira J. Robinson
Junior Biology Health Science Major
Tennessee Technological University
April 27, 2005
*Project Summary
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To see what type of bacteria and e-coli grows in
different teas
To see how many coliforms grow from tea samples
Take several different tea samples and run tests
Take a count of how many coliforms were present
at the end of the experiment.
Give data and charts on results
Introduction
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There have been several media reports on the health hazards of fecal
coliform contamination of restaurant brewed iced tea ( Doyle 1996).
In January the CDC issued an alert stating that ice tea can breed
bacteria if it sits out all day (Kapner 1996).
There may be microbes, but they probably are of the relatively
harmless variety that naturally cling to tea leaves (Nutrition 1996).
“The Centers for Disease Control and Prevention, which investigated
the reports about iced tea, explained that theoretically tea could
transmit disease if other bacteria survived the brewing (Iced 1996).
The large numbers of relatively benign bacteria that showed up
probably occurred because of lax sanitation of restaurant tea dispensers
(Nutrition 1996).
Objective Statement
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The objective for this experiment is to se
what type of bacteria and microorganisms
grow in different tea samples, if in they do,
whether e-coli or metallic sheen is present.
Hypothesis Statement
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I hypothesize that microorganisms do grow
in tea samples due to the tea leaves and or
the amount of time tea is left sitting.
Methods & Materials
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Pour Plate method on TSA & Endo Agar Plates
Each plate will have a different measurement of
.1, .5, 1 mL
Coliform count
Incubation of the plates for growth
Tea Samples from 3 different restaurants
Data will be recorded in tables
This experiment will be administered by Dr. Goss
Results
Endo Agar Plate Results
 After incubation I found in restaurant A1 that it
contained metallic sheen with fecal colifroms,
which is e-coli.
 All of restaurant A had grown mold
 B1 also contained metallic sheen and fecal
coliforms.
 C1 only contained metallic sheen
Results Cont.
TSA plate results
• All of the 3 restaurants contained fermentation
• See Chart For More Details
Data
Endo Agar Plates
mL.
Restaurant A
Restaurant B
Restaurant C
.1
58 colonies
252
372
colonies
colonies
54 colonies
500
Ex.
1
2
.5
29 colonies
colonies
3
1
121
26 colonies
colonies
710
colonies
Comments: A1 contained metallic sheen with fecal coliforms. A2 contained only fecal coliforms (e-coli). All of A had mold.
B1 contained metallic sheen and fecal coliforms.
C1 only contained metallic sheen.
TSA Plates
mL.
Restaurant A
Restaurant B
.5
17 colonies 9 colonies
Restaurant C
Ex.
1
400
colonies
2
1
13 colonies 14 colonies 800
colonies
Comments: All restaurant samples contained fermentation
Pictures
Literature Cited
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Doyle, Michael. Food Technology. Fecal conditions in tea:
what’s the problem. Oct. 1996 v50 n10 p104(1)
Environmental Nutrition. Iced tea scare a tempest in a
teapot. June 1996 v19 n6 p8(1)
Environmental Nutrition. Iced tea scare a tempest in a
teapot. June 1996 v19 n6 p8(2)
Kapner, Suzanne. Iced-tea ‘brewhaha’: CDC says drink
OK. June 1996 v30 n23 p1(2)
Marcinkowski, Victoria. Coffee, tea, or bacteria? Jan. 2003
v59 i8 p6(2)
Conclusion
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There are bacteria present in tea.
 For the most part it isn’t harmful
 Some of the causes can be from the tea
leaves and or the time it has sat in high
temperatures.
 The growth of e-coli can be harmful
 You can keep drinking tea!!!!!
Acknowledgements
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I would like to thank Dr. Goss for her time
and help
 A special thank you to Cara Sanders for an
outstanding hand.
 A special thanks to Kimberly Tuggle for
being a great partner.
 Thank you Dr. Morgan for all the help
ANY QUESTIONS????
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Please Don’t ASK
ANY!!!!!!!!