Biochemical Pathways: Eduard & Hans Buchner
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Transcript Biochemical Pathways: Eduard & Hans Buchner
By: Haben M. & Cordelia E.
BACKGROUND
These 2 brothers were born in Munich,
Germany
Their father was a professor of forensic
medicine
Hans was the oldest of both
He was a physician who researched
blood proteins that can destroy bacteria
Eduard: chemist; researched yeast cells
along side Adolf von Baeyer
What was already known?
That food spoiled after a few days when
exposed to air
The fermenting agent comes from the air
The cause: bacteria
The theory of fermentation was first
developed by Louis Pasteur
He believed that fermentation was
physiological in yeast cells
In contrast Justin von Liebig, a chemist,
believed that the cause was a chemical
agent within the cell
The Experiment:
Hans Buchner worked along side his
younger brother by contributing the
study of anaerobic bacteria
He discovered the blood serum could kill
bacteria,
But this ability was useless if heated
Anaerobic bacteria: bacteria that can
exist with limited or complete absence of
oxygen
Summarize:
Eduard Buchner used a pestle & mortar to
ground up yeast
Then extracted juices
The "dead" liquid fermented a sugar
solution, which formed carbon dioxide &
alcohol much like living yeasts.
The “non-living“ yeast behaves just like the
cells
It was then acknowledged that
fermentation is caused by an enzyme
Cell-free Fermentation:
Buchner continued Pasteur’s
discoveries
Through a series of experiment he
discovered the enzyme: ZYMASE
This enzyme catalyzes the reaction of
fermentation to produce C2H5OH and
CO2
Proved that yeast cells were not needed
Relation to cellular respiration:
Ferments glucose
Produces ethanol and carbon dioxide in
the pyruvate stage
Fermentation is an added cycle that
replaces the Krebs cycle
There is no need for oxygen
What was learned?
Zymase catalyses the breakdown of
glucose in the process of glycolysis
Eventually slows down the process
Yeast can breakdown sugars with or
without oxygen
Enzymes can be extracted from yeast
Final products: alcohol & carbon dioxide
References:
Eduard Buchner. (n.d.). Retrieved November 15,2010 from
http://www.nndb.com/people/227/000099927/
"Eduard Buchner - Biography". Nobelprize.org. 16 Nov 2010
http://nobelprize.org/nobel_prizes/chemistry/laureates/1907/buch
ner-bio.html
Cell- free fermentation. (n.d.). Retrieved November 15,2010 from
http://nobelprize.org/nobel_prizes/chemistry/laureates/1907/buch
ner-lecture.pdf
The background to Eduard Buchner's discovery. (n.d.) Retrieved
November 15, 2010 from http://www.jstor.org/pss/4330549
Zymase. (n.d.). Retrieved November 15, 2010 from
http://www.answers.com/topic/zymase
Eduard Buchner. (n.d.). Retrieved November 15,2010 from
http://www.britannica.com/EBchecked/topic/82903/EduardBuchner
Cellular Respiration & Fermentation. (n.d.). Retrieved November
16, 2010 from
http://biology.clc.uc.edu/Courses/bio104/cellresp.htm
THE END