Transcript Microscope Slide Show - Garnet Valley School District

Chapter 1 continued.......
…… Microscopes
(It would be difficult to
study our cells and
bacteria cells if we could
not see them!!!)
The specimen scientists want to look at
determines the types of microscopes they will use.
Types of Microscopes:
1. Compound Light Microscope
- Uses light to view specimen
- Uses 2 lenses: ocular and
objective lenses to focus and
magnify specimen
- Used for viewing live or dead
thin specimens, can see a
single cell.
- Magnifies up to ~1000x

Chloroplasts in plant cells
Figure 4.5Bx2
2. Stereoscope or Dissecting
Microscope
- Uses light to view specimen
- Gives a 3-D image
- Can be used to view larger
live or dead specimens
- Not capable of high
magnification
Stereoscope/Dissecting Microscope
tp://faculty.clintoncc.suny.edu/faculty/Michael.Gregory/files/Bio%20101/Bio%20101%20Laboratory/microscopy/dissecting_scope2.jpg
Sea lice on the skin of Atlantic
salmon (Salmo salar L.) 5x
Sea lice on the skin of Atlantic salmon (Salmo salar L.)
http://www.marlab.ac.uk/Uploads/Images/Sealice.jpg
3. Electron Microscopes
- Uses a beam of electrons to
view specimen
- Capable of high magnifications
- Uses electromagnets to magnify
and focus image
- Can only view dead
specimens!!!!
Photos of bacteria from SEM and TEM
TEM
SEM
http://www.karlloren.com/biopsy/images/TEM-Fission_rod.jpg
http://chem.ch.huji.ac.il/~eugeniik/biofuel/biofuel_cells_bacteria4.jpg
-There are 2 types:
- SEM: Scanning Electron
Microscope
- Scans the surface of
the specimen
- Gives a 3-D image of
a whole specimen

Scanning
electron
microscope
(SEM)

Scanning
electron
micrograph of
cilia
Figure 4.1B
Copyright 2003 Pearson Education, Inc as Benjamin Cummings

Transmission
electron
microscope
(TEM)

Transmission
electron
micrograph of
cilia
Figure 4.1C
Copyright 2003 Pearson Education, Inc as Benjamin Cummings
- TEM: Transmission Electron
Microscope
- Sends a beam of
electrons through a
thin specimen.
- Gives a 2-D image
of internal cell parts.

Prokaryotic cells, Bacillus polymyxa
Figure 4.4x1
Copyright 2003 Pearson Education, Inc as Benjamin Cummings