Transcript Mouthwash Effectiveness Against Oral Bacteria David A

Mouthwash Effectiveness Against Oral Bacteria
David A. Adams Microbiology Undergraduate - Tennessee Tech University Cookeville, TN 38505
Introduction
Results
•Undesirable oral odors generally come
from proteins and peptides hydrolyzed
by gram-negative bacteria (McDowell
1993)
•Bacteria in the mouth are primarily
responsible for gingivitis (Journal of
American Dental Association 1998)
•The number of viable bacteria dropped
by 94% after rinsing with antiseptic
mouth rinse (Fine et al 1993)
Discussion
•Staphylococcus was the least
resistant bacteria
•Pseudomonas and Escherichia were
equally resistant
•Better results may be obtained by
testing a greater number of different
oral bacteria
Staphylococcus
Water
Mouthwash A Mouthwash B Mouthwash C
Inhibition Zone mm
0
Trial 1
Trial 2
Trial 3
Average
0
0
0
0
17
14
16
16
14
10
13
12
18
18
17
18
Escherichia
Water
Inhibition Zone mm
Trial 1
Trial 2
Trial 3
Average
Objective/Hypothesis
Mouthwash A Mouthwash B Mouthwash C
0
0
0
0
8
8
8
8
8
7
7
8
9
8
8
8
Pseudomonas
Water
Objective
The objective of this research was to
determine the susceptibility of bacteria
to different brands of mouthwash
Inhibition Zone mm
Trial 1
Trial 2
Trial 3
Average
Conclusion
Mouthwash A Mouthwash B Mouthwash C
0
0
0
0
8
7
8
8
7
8
7
7
•All 3 mouthwash brands inhibited
bacterial growth
•Some bacteria are more resistant to
antiseptics than others
•The generic brand mouthwash,
Equate, was the most effective
•More cost does not always equal
better value
8
9
9
9
Mouthwash Comparison
Hypothesis
Rinsing with mouthwash will have no
benefit over rinsing with water to
prevent bacterial growth.
18
18
16
16
Methods and Materials
•Methods were derived from Dr.
Goss’ 2004 Medical Micro lab
manual
•3 TSA plates inoculated with 3
different oral bacteria for 24 hours
•Three individual mouthwash brands
were tested
•Water used as control (D)
•Performed three trials & took
average
Inhibition Zone (mm)
14
12
12
10
8
8
8
9
8
7
4
Acknowledgements
8
6
0
0
2
Staphylococcus
0
0
Pseudomonas
Water
Scope
Escherichia
Listerine
Equate
Escherichia
Pseudomonas
Staphylococcus
My utmost thanks go to Dr. Goss,
who helped to make this research
possible. I would also like to thank
the Tennessee Tech Biology
Department for allowing me to use
their tools and facility.