Transcript torelance level of different cabbage varieties to black rot

TORELANCE LEVEL OF DIFFERENT
CABBAGE VARIETIES TO BLACK ROT
BY: MUNENE DAVID M.
A22/0081/2009
SUPERVISOR: PROF. DANIEL MUKUNYA
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Cabbage is the most valued and the most used vegetable
in the world
Of all the crucifers, cabbages are the most affected by
black rot caused by a bacteria Xanthomonas campestris
pv campestris
Different seed companies have breed for resistant
varieties (F1) but farmers are still suffering losses related
to black rot
This means there is a need to evaluate the most resistant
variety that can minimize losses encountered by the
farmers around Kabete
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Despite breeders effort to breed for resistant varieties of
cabbage, black rot disease has remained to be a threat in most
parts of the world, especially in small scale
Production(21MT/ Ha) has remained much lower than the
yield potential (26-75 MT/Ha depending on variety) despite
high demand, good market prices and health benefits
associated with cabbages
Infected cabbages are also prone to other pathogens leading to
high storage losses
This is as result of break down of resistant gene following the
continued mutation of the bacteria over time
General objective;
 Evaluation of the most tolerant cabbage varieties against black
rot commonly grown in Kenya
Specific objective;
 Determine the incidence of black rot in different cabbage
varieties
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Four cabbage varietiesRiana, Fanaka F1, Pructor
F1, Queen F1
1 kg DAP
2kg CAN
pesticides
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70% alcohol(100ml)
3% Sodium hypochlorite
(50ml)
Sterile distilled water
Empty Petri dishes
12 plates of nutrient agar
Wire loop
Spirit lamp
12 universal bottles
Clean distilled water
Teat pipette/ dropper
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Planting and establishment of cabbages in the field
Collection of young black rot infected cabbage material
from the field station for bacterial isolation in the laboratory
Isolation of bacteria using aseptic method
Sub-culturing of bacteria to produce clean pure colonies
Serial dilution to achieve different numbers of colony
forming units(c.f.u)
Bacterial inoculation to cabbage plants
Data collection
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Bacteriology exercise book, fourth year, isolation of bacteria
http://go.warwick.ac.uk/wrap
Edward J. Sikora, Extension Plant Pathologist, Professor, Entomology and
Plant Pathology, Auburn University
International Rules for Seed Testing Annexe to Chapter 7: Seed Health Testing
Methods
HCDA ( Horticulture Crop Development Authority), 2007 Report
http://dx.doi.org/10.1094/PDIS-94-3-0298
Massomo SMS, Mabagala RB, Swai IS, Hockenhull J, Mortensen CN .
“Evaluation of varietal resistance in cabbage against the black rot pathogen,
Xanthomonas campestris pv. campestris in Tanzania.” Crop Protection
23,4(2004): 315-325.
Chupp C. “Black rot of cabbage.” Manual of Vegetable Plant Diseases. New
Delhi, India : Discovery Publishing House, 2006. p. 132-133
Williams PH. "Black rot: a continuing threat to world crucifers." Plant
Disease 64.8 (1980): 736-742.
"Black rot of cabbage and other crucifers." Integrated Pest Management.
University of Illinois Extension. Dec 1999.
Activity
Raising
seedlings in the
nursery
watering
Transplanting
gapping
Top dressing
Data collection
proposal
inoculation
Data analysis
Final
presentation
Dec
Jan
Feb
March
April
May
THANK YOU FOR YOUR AUDIENCE