Transcript Service training part 1

Introduction to training
Agenda
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Product overview
What’s new from ProStar 325
Hardware overview
Initialization routine
Software CD overview
Detector installation & PolyVerfiy
PolyDiagnostics
Product Overview
Based on the ProStar 325 with many shared parts
Two models available:
• Single 1024 array
• Dual 1024 array
Control by both Star with PolyView 2000 and Galaxie
(PolyView is now included with Star driver)
Product Overview - workstation control
Galaxie
• Four 335 DADs per acquisition server
• Driver included in Varian LC control drivers
Star
• two 335 DADs per Star Workstation
• Driver included in LC control option
• Installation of driver automatically installs PolyView
2000
Ethernet communications best with a switch (not a hub)
Product Overview
ProStar 335 and 325 similarities
• Same cabinet
• Same UV & visible lamps
• Same flow cells
• Same rear panel, connectors, interface boards & cables
• Same communications protocol
• Same power supply
• Same Main pwb (difference in firmware)
What’s New
Same look as ProStar 325 except
no PPC capabilities
What’s New
Different optics module
Dual Array
• 1024 photodiode sample and reference array
• Extended range spectra
• Used for Analytical to Prep Applications
Single Array
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1024 photodiode sample array
2 photodiode references (250 and 500 nm)
No extended range
Uses 9x0 and 4x0 flow cells only
Hardware Overview – optics module
Product Overview – ray trace diagram
Hardware Overview - Polychromator
Available
slit widths
1nm
2nm
4nm
8nm
16nm
Hardware Overview – dual path flowcell
Illustration is of a 9x1
flowcell
reference
sample
Hardware Overview – optics module
Wavelength calibration data must be entered into the
EEPROM using Diagnostics software.
Electronics – wavelength calibration factors
Use the floppy disk to
update the EEPROM
automatically
This label will be
attached to the top of
each optics module
Hardware Overview – Electronics
Only difference between 335 & 325 is firmware
Hardware Overview - Electronics
Add new MAC address
label when swapping pwb’s
New MAC address
label comes with
each new PWB
Hardware Overview – initialization routine
• Peak search for 656.1nm deuterium emission line
• Determines dark current table (0%T)
• Gain (Lamp) calibration is now user driven. Stored in
non-volatile memory.
Initialization
initialized
Hardware
fault
not initialized
Status LED’s
Software CD overview
Software CD ships with each ProStar 335 detector.
Part number: 85 102114 00
Auto-loading CD which contains:
• Galaxie drivers and manuals
• Star driver and manuals
• PolyVerfiy software
• PolyDiagnostics
Software CD overview
Customer software CD 851xxxxx-00 will contain
Software CD overview – installation PolyVerify
After installing, a
desktop icon will
be created called
ProStar
Use PolyVerify to check the
performance of the ProStar 335
Software CD overview - installation PolyVerify
Only two test are
required at
installation;
Noise/Drift
Wavelength
accuracy
IQ/OQ is also available
part number: 03-914756-00;4
Software CD overview – installation
PolyVerify
Print customer certificate
The ProStar 335 must be initialized and have both
lamps on for at least one hour prior to running any tests.
Noise test : pump water for at least 10 minutes
at a pump head pressure of at least 100 atm
using a back pressure restrictor of 40psi.
Wavelength accuracy: same conditions as the
Noise test.
Software CD overview - PolyDiagnostics
Installing PolyDiagnostics
Browse the CD using
Windows Explorer.
Diagnostics is not self
loading and not intended for
customer use.
Software CD overview - PolyDiagnostics
To run PolyDiagnostics you will need to copy these three
files to the ProStar directory where PolyVerify is installed.
From there double click the “PolyDiagnostics.exe” file
using Windows Explorer.
Software CD overview - PolyDiagnostics
Similar
appearance to
325 Diagnostics
Software CD - PolyDiagnostics
Use the Diagnostics program to update the EEPROM
and perform various calibration routines
i.e. wavelength & lamp calibrations
Service folder also contains the
firmware updater program
Software CD - PolyDiagnostics
The EEPROM contents will need updating whenever;
• the Main PWB is replaced
• the optics module is replaced
• to update installation & maintenance dates
Software CD - PolyDiagnostics
EEPROM contents;
• Detector serial number
• MAC address
• Wavelength calibration factors, slope and offset
• IP address
• Gateway address
• Subnet mask
• Lamp timer (on/off date and times)
• Detector installation date
• Last maintenance date
• Error log
• Hg wavelength calibration date
Software CD - PolyDiagnostics
When to calibrate
Performing a Lamp Calibration will help reduce noise and
improve linearity.
• Deuteriunm lamp is changed
• Visible lamp is changed
• Every 1000 lamp source hours
• Increasing signal noise
• When changing flowcell to one of a different pathlength