Transcript File

SRC
Protocol Clarification
Acronyms
• BSL
– Bio Safety Level
• FTQ
– Failure to Qualify
Known source to culture
1. BSL-1
2. BSL-2
3. No BSL classification
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3
Okay for Juniors?
1. Yes
2. No
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2
Known BSL 1 source to subculture
1. BSL-1
2. BSL-2
3. No BSL classification
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2
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3
Okay for Juniors?
1. Yes
2. No
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0%
2
Unknown source to culture
1. BSL-1
2. BSL-2
3. No BSL classification
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1
0%
2
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3
Okay for Juniors?
1. Yes
2. No
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0%
2
Unknown source to standard plate to
subculture
1. BSL-1
2. BSL-2
3. No BSL classification
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1
0%
2
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3
Okay for Juniors?
1. Yes
2. No
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0%
2
Unknown source to selective media
to subculture
1. BSL-1
2. BSL-2
3. No BSL classification
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0%
2
0%
3
Okay for Juniors?
1. Yes
2. No
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2
Unknown source to culture to slide
1. BSL-1
2. BSL-2
3. No BSL classification
0%
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0%
2
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3
Okay for Juniors?
1. Yes
2. No
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2
BSL 2 source to standard plate
1. BSL-1
2. BSL-2
3. No BSL classification
0%
1
0%
2
0%
3
Okay for Juniors?
1. Yes
2. No
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0%
2
BSL 2 source to standard plate to
subculture
1. BSL-1
2. BSL-2
3. No BSL classification
0%
1
0%
2
0%
3
Okay for Juniors?
1. Yes
2. No
0%
1
0%
2
Known BSL 1 source to plate to
subculture, source provided to student
1. BSL-1
2. BSL-2
3. No BSL classification
0%
1
0%
2
0%
3
Okay for Juniors?
1. Yes
2. No
0%
1
0%
2
Known BSL 2 source to plate subculture,
source provided to student
1. BSL-1
2. BSL-2
3. No BSL classification
0%
1
0%
2
0%
3
Okay for Juniors?
1. Yes
2. No
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0%
2
Lyophilized BSL1 source to standard plate
1. BSL-1
2. BSL-2
3. No BSL classification
0%
1
0%
2
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3
Okay for Juniors?
1. Yes
2. No
0%
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0%
2
Lyophilized BSL1 source to standard plate
to subculture
1. BSL-1
2. BSL-2
3. No BSL classification
0%
1
0%
2
0%
3
Okay for Juniors?
1. Yes
2. No
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1
0%
2
Lyophilized BSL1 source to hydrating broth
to nutrient cuvette, sealed and incubated.
1. BSL-1
2. BSL-2
3. No BSL classification
0%
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0%
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3
Okay for Juniors?
1. Yes
2. No
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0%
2
Bread molds
1. BSL-1
2. BSL-2
3. No BSL classification
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Okay for Juniors?
1. Yes
2. No
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0%
2
Bread really molds
1. BSL-1
2. BSL-2
3. No BSL classification
0%
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3
Okay for Juniors?
1. Yes
2. No
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0%
2
Slime mold to filter paper and feed
1. BSL-1
2. BSL-2
3. No BSL classification
0%
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3
Okay for Juniors?
1. Yes
2. No
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0%
2
Slime mold to plate and not opened to
feed
1. BSL-1
2. BSL-2
3. No BSL classification
0%
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0%
2
0%
3
Okay for Juniors?
1. Yes
2. No
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0%
2
Slime mold to plate and re-open to feed
1. BSL-1
2. BSL-2
3. No BSL classification
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3
Okay for Juniors?
1. Yes
2. No
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2
Ballistics project, certified officer supervises
1. FTQ
2. No FTQ
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Ballistics project, dad supervises
1. FTQ
2. No FTQ
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Archery project, dad supervises
1. FTQ
2. No FTQ
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Archery project, certified expert supervises
1. FTQ
2. No FTQ
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Lasers
Student collects ocean (or pond) water. Student wants to compare
different fertilizers' effects on plankton growth.
They set up containers at home, add fertilizer, organic fertilizer and
manure to the containers.
1. BSL-1
2. BSL-2
3. No BSL classification
Would it make a difference if
manure was not used?
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A student surveys Alzheimer's patients at a day care facility. They have
all the proper paperwork, and have a qualified scientist who has
a masters degree in library science, but is a technician in an
Alzheimer's research unit at a research hospital.
1. FTQ
2. No FTQ
What if the qualified scientist had been
appropriate? (I.E. had came from
background in Alzheimer’s care)
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A student obtains oil-eating bacteria and grows them in petri dishes
with different types of oil to look at efficacy of the bacteria on
different weights of oil.
Student does not open the petri dishes.
1. BSL-1
2. BSL-2
3. No BSL classification
PHBA--must be done in a BSL-1 lab.
Oil-eating bacteria are only exempt
from being considered PHBA
projects if not cultured
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Student wants to study the effectiveness of different products at
repelling deer. His back yard is adjacent to a conservation area, so he's
going to do this at home.
1. FTQ
2. No FTQ
•Vertebrate project--requires vertebrate forms.
•Student will have to find some way to report mortality/assure no more than
15% weight loss to the animals
•Essentially they will not be able to do this, especially if they are using some
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home-made products in their treatments.
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