Transcript Trichomoniasis - National Institute for Animal Agriculture

Trichomoniasis
Field Perspectives
Jeremy VanBoening, DVM
Trichomoniasis Involvement
• Clinical Practice
• Nebraska Cattlemen-NCBA
• Nebraska Veterinary Medical Association
Sample Collection
Texas A&M Trich Sample Collection Video
Sample Collection Challenges
Survey of 10 D-Labs 10-14-2013
Do the samples need to be
incubated?
Lab ID
Do the samples need to be put on ice
when shipped?
How many samples can be pooled?
Preferred collection medium?
A
Yes 24hrs
Yes
Up to 5
tubes
B
No
No, dark and warm packaging
Up to 5
InPouch
C
No, room temp
No
Up to 5, but not recommended
InPouch
D
You can if you want, just state on
submission form if you incubated
samples or not. Have to receive
samples within 72hrs of collection.
If cold outside put a thawed ice pack. If
hot outside put ice pack but not directly
on samples
E
No, room temp, ship same day
No
No pooling
InPouch
F
Yes or No, state on form if you did.
Has to arrive at lab within 120hrs
after collection.
No
Up to 5
InPouch
G
No
cold pack
Up to 5
Scraping in 5ml of Saline placed in tube.
H
I
No
No
Up to 5
InPouch
No
No, room temp
No pooling
InPouch
J
No, ship overnight
Yes
Up to 5
Up to 3
InPouch or tubes
wash in red top tube with saline
Summary of PCR Related Questions
Incubation
Yes= 2
No=6
Your Choice=2
Ship on Ice
Yes=2
Room Temp=6
Other=2
Pooling
Up to 5=7
No Pooling=2
Up to 3=1
Preferred
Collection Media
In Pouch=6
Saline=2
Tubes=2
Sample Collection Challenges
Restraint and Safety
Is this safe??
What Collection Method
1.
2.
3.
4.
preputial scraping with pipette
preputial scraping using thrichit
saline rinse
swap or scraping of the penis
NO BLOOD for Either PCR or Culture
Sample Collection Challenges
Cleanliness
 Field Collection Area
 Scissors (Disinfect or Not and with what?)
 Access to saline for rinsing
Dirty Samples
• Rarely if Ever Return Positive
• Recent Study
Hustun, etal. MSU
– 20 Samples w/ bacteria injected at increasing #’s
– 20 Samples clean (Only T. Foetus Added)
– 0 of 20 with bacteria ever recorded as positive
– 20 of 20 w/out bacteria had at least one positive
recording
Sample Collection Challenges
Proper Collection Media
Sample Preparation
 Depends on the D-Lab!!
 Collection Medium (In-Pouch, Tubes, Saline)
 Incubate or Not
• Depends on D-Lab, PCR or Culture,
 How should the sample be packaged?
• On Ice, Room Temperature, Or Depends on the
Weather
Transportation Issues
• Rural Access to Timely Reliable Transportation
• How to package and send samples?
– How long can they be in transit and at what
temperature?
– Sensitivity and Specificity go down for both
culture and PCR
Hustun etal MSU
How Do We Get Better????
Standardization
1. Adopt the Best Diagnostic Testing Available
(qPCR) Using Chemical-Lysis and IAC.
2. Promote SOP’s for Sampling, Sample Prep, and
Shipping
3. Increased Veterinarian/Producer Awareness
4. Standardized Regulations