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Sequence Quality 12000 10000 4000 Up to 1/3 of sequences from a clone library are putative chimeras. 6000 2000 isolate 1000 1000 4000 900 WA CC WA FC GI SO putative chimera WA CC WA SO SO IN WA GI CC AR SO VG GI GI SO WA WT CC GI GI GI 8000 2000 clone 700 -1000 600 0 400 WA isolate clone CC WA FC GI SO WA CC WA SO SO IN WA GI CC AR SO VG GI GI SO WA WT CC GI GI GI 500 -2000 isolate cloneputative chimera putative chimera -3000 300 200 WA CC WA FC GI SO WA CC WA SO SO IN WA GI CC AR SO GI VG GI SO WA WT CC GI GI 0 Sequence Source Comprehensive chimera screening of public data using newlydeveloped Bellerophon3. Provides quality-controlled set for probe selection. 100 GI Sequence Count 8000 3000 Taxonomy in Flux • • • • Incongruent taxonomic nomenclature even at phylum level. Making multiple taxonomic classifications available through Greengenes will aid in standardizing classification, particularly for environmental lineages. Greengenes integrates each, allowing a balanced approach to nomenclature of newly discovered organisms. EXAMPLE: Halanaerobium salsugo Hugenholtz=Bacteria; Haloanaerobiales; Haloanaerobiaceae; Haloanaerobium/ Haloincola Ludwig=Bacteria; Haloanaerobiales; Haloanaerobiacae; Haloanaerobium Pace=Bacteria; Haloanaerobiales; Haloanaerobiaceae; Haloanaerobium/ Haloincola RDP=Bacteria; Firmicutes; Clostridia; Halanaerobiales; Halanaerobiaceae; Halanaerobium NCBI=Bacteria; Firmicutes; Clostridia; Halanaerobiales; Halanaerobiaceae; Halanaerobium Each yellow dot represents a named phyla Only a fraction of the phyla are recognized by all five major curators. Probe/Primer Design Check phylogenetic scope of a primer or probe. Web-accessible repository for DNA microarray data. Password protected Database is being developed to allow authorized users to search for bacteria of interest by city or by time (date). Weather data corresponding to the collection dates will be linked. Given that there are over 9,000 OTUs detectable per chip, the database will provide information on more than 2 million data points that can be viewed in relation to weather patterns. Example of how bacterial groups can be mapped to geographic location. QuickTime™ and a TIFF (LZW) decompressor are needed to see this picture. D1D2 analysis • 12,422 aligned D1D1 regions are in our database • representing 1909 fungal taxa (according to annotation distributed by NCBI • 5,488 OTUs were found. • 2,283 OTUs had 1 or more great probes • 3,801 OTUs had a distributed solution • 4,828 OTUs have probes that could all be triggered by <= 5 other OTUs