Transcript Bacterial Growth Calculations Presentation

Bacteria Colonies on Culture Plate
Time, warmth, oxygen
Flask filled with liquid bacteria food
Bacterial growth leads to darker liqu
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# Bacteria
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Exponential Bacterial Growth
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Light
Prism
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Detector
Computer
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For optimal protein production from
bacteria, a scientist wants to grow the
bacteria to a density (OD600) of 2.5-3.5.
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Once this density of bacteria has been
achieved, the scientist will give the bacteria
a chemical “kick” known as induction.
This will cause the bacteria to start
producing the protein of interest.
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We now want to continue to grow the
bacteria for an additional two hours after
the induction “kick.”
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