Transcript No Slide Title

1. Prepare RNA mix in PCR tube
1. 1 µg RNA
2. 1 µl Random primer/poly dT mix
3. 1 µl 10 mM dNTP
4. Water to 12 µl
2. Leave 5’ @ 65˚ C, then chill to 4˚ C
3. Add
1. 4 µl 5x first strand buffer
2. 2 µl 100 mM DTT
3. 1 µl RNAse inhibitor
4. Leave > 2’ @ RT
5. Add 1 µl Superscript III
6. Leave 10’ @ 25 ˚ C, then 50’ @ 42 ˚ C
7. Inactivate by leaving 15’ @ 70˚ C
8. Use 1 µl for PCR with gene-specific primers
1. Set up master mix for each primer combo on ice!
1. 1.1 µl 100x F primer (1 pMol/µl = 1µM final [])
2. 1.1 µl 100x R primer
3. 11 µl 10x PCR buffer
4. 2.2 µl 10 mM dNTP (200 µM final [])
5. 88.4 µl water
6. 0.7 µl Taq polymerase
2. Primers are
1. corn SOD or PEPC
2. Rice OEE or PHYA
3. Arabidopsis Actin and Aromatic Acid Decarboxylase
3. Add 19 µl to 1 µl correct cDNAs, 19 µl to 1 µl correct
genomic DNA, 19 µl to 1 µl no template cDNA and 19 µl
to 1 µl water
4. Run 30 cycles of 15” @ 94, 50-1”/cycle @ 50, 15” @ 72
Possible Projects
1. Studying interactions between plants
and Geobacter (or other electrogenic
bacteria)
Possible Projects
1.Studying interactions between plants and Geobacter (or
other electrogenic bacteria)
2.Studying interactions between
cyanobacteria and Geobacter
(or other electrogenic bacteria)
Possible Projects
1. Studying interactions between plants and Geobacter (or
other electrogenic bacteria)
2. Studying interactions between cyanobacteria and
Geobacter (or other electrogenic bacteria)
3. Studying potential of Rhodopseudomonas (a.k.a.
Rhodobacter) for biophotovoltaics
Possible Projects
1.Studying interactions between plants and Geobacter (or
other electrogenic bacteria)
2.Studying interactions between cyanobacteria and
Geobacter (or other electrogenic bacteria)
3.Studying potential of Rhodopseudomonas (a.k.a.
Rhodobacter) for biophotovoltaics
•Switch to photosynthesis when go anaerobic!
Possible Projects
1.Studying interactions between plants and Geobacter (or
other electrogenic bacteria)
2.Studying interactions between cyanobacteria and
Geobacter (or other electrogenic bacteria)
3.Studying potential of Rhodopseudomonas (a.k.a.
Rhodobacter) for biophotovoltaics
•Switch to photosynthesis when go anaerobic!
•Selection for cells that can transfer e- to electrodes!
1.
2.
3.
4.
Possible Projects
Studying interactions
between plants and
Geobacter (or other
electrogenic bacteria)
Studying interactions
between cyanobacteria
and Geobacter (or other
electrogenic bacteria)
Studying potential of
Rhodopseudomonas
(a.k.a. Rhodobacter) for
biophotovoltaics
Studying Cyanobacteria
for biophotovoltaics
Possible Projects
4. Studying Cyanobacteria
for biophotovoltaics
• Finding new ones
Possible Projects
4. Studying Cyanobacteria
for biophotovoltaics
• Finding new ones
• Tweaking knowns
• http://www.invitrogen.co
m/site/us/en/home/Produ
cts-andServices/Applications/Pr
otein-Expression-andAnalysis/ProteinExpression/algaeengineering-kits.html
Possible Projects
4. Studying Cyanobacteria
for biophotovoltaics
• Finding new ones
• Tweaking knowns
• Tweaking nanowires
Possible Projects
5. Studying green algae for biophotovoltaics
• Finding new ones
• Tweaking knowns (Chlorella, Dunaliella)
Possible Projects
6. Studying plants for biophotovoltaics
• Roots under hypoxia
Possible Projects
6. Studying plants for biophotovoltaics
•Roots under hypoxia
•Aquatics under CO2 deprivation
Possible Projects
6. Studying plants for biophotovoltaics
•Roots under hypoxia
•Aquatics under CO2 deprivation = every day!
Possible Projects
6. Studying plants for biophotovoltaics
•Roots under hypoxia
•Aquatics under CO2 deprivation = every day!
•Some aquatics do CAM because CO2 is low in water in the
day
Possible Projects
6. Studying plants for biophotovoltaics
•Roots under hypoxia via mediator
•Aquatics under CO2
deprivation
•Some aquatics do CAM
because CO2 is low in water
in the day
•Perhaps others will
transfer electrons to anodes
during the day to dissipate
excess energy
Possible Projects
7. Engineering the production of other novel products