Transcript WATERS Laboratory

CWRS Advisory Board Meeting
March 1, 2007
Gretchen Grover – Chemist
Christal Wade - Microbiologist
Rick Fowler – Environmental Biotechnologist
WATERS Employees
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Director: Jana Fattic
Lab Analysts:
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Microbiology Department: Rick Fowler, Christal Wade
Chemistry Department: Gretchen Grover, Dong Li
Office Associate: Kristy Mayes
Students: Samantha Kramer, Rebekkah Lively, Jennifer
Dean
Customers
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Private
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Wastewater
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Total Coliform/E.Coli
Oil Wells
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COD, Oil and Grease, TDS, TSS
Drinking water
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BOD, pH, TRC, DO, TSS, Fecal Coliform, Ammonia Nitrogen
Storm water
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Total Coliform/E. Coli, Metals, Ions, Atrazine
pH, TDS, Specific Gravity, Chloride
Research Projects
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TOC, Atrazine, Metals, Ions, DNA
Certifications
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Drinking water Certification
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Inorganic Certification
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June Audit
Cryptosporidium
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January Audit
Long Term 2 Enhanced
Surface Water Treatment Rule
(LT2)
Accept outside samples under
LT2 and provides field
filtration apparatus
www.epa.gov/safewater/disinfection/
LT2/lab_aprvlabs.html
Chemical Hygiene Plan
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Chemical Hygiene Officer
Projects
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Mammoth Cave
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City of Bowling Green
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River & Cave System monitoring
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Flood Pulse Event
Watershed Watch
Upper Green River watershed monitoring (E.coli)
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Analyzing samples for this project
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TMDL
University Research
Support WKU faculty as well as other university projects
Quality Assurance
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Continued revision and updating of SOP to
the 21st Edition Standard Methods
QA/QC Improvements
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New Standards
PT/QC (quarterly)
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USGS
APG
Important Purchases
Chemistry
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Accumet excel XL60
 TKN Digestor
 Konelab
Microbiology
Bench top water bath
FastGel electrophoresis apparatus
 PCR Workstation
 Qubit fluorometer
 Microcentrifuge
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Where do we go from here?
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Current Capabilities
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Cryptosporidium
Konelab Analyses
Drinking water, Wastewater, Storm water analyses
Bacteriological/DNA analyses
Future Capabilities
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Unregulated Contaminant Monitoring Rule (UCMR2)
Organics (Work toward a GC MS/MS and HPLC)
Radionuclides
Direct a Chemical Laboratory Certification Program for
Kentucky
Voluntary Wastewater Certification
Where do we go from here?
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New customers bring more cash flow
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11 new customers
Networking; customer surveys
Pick up service
Consultation services
Sample collection services
Microbiology
Programs
Christal Wade
Drinking Water Microbiology
Laboratory Sample Load
Sample Type Analyzed Number Analyzed/year
Drinking Water (Public)
2080
Drinking Water (Private)
25
Wastewater
0
Surface (raw)
745
Total
2850
Completed latest On-Site Audit in January 2007
EPA Approval for Crypto
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WATERS Lab received approval from EPA
for the analysis of Cryptosporidium on
October 18, 2006.
Currently have a cooperative agreement
with Appalachian States Analytical, L.L.C.
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Total of 20 surface water clients
Will be starting monitoring in June
4 Contracts with other PWSs
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From both Kentucky and West Virginia
Several quotes outstanding
Latest PT Results
From September 2006
EPA Protozoan Proficiency Testing Laboratory Results Summary
Round: 0906
Lab ID: KY00002
Filter: Envirochek HV
Cryptosporidium
Sample ID
Oocysts
Reported
Oocysts
Spiked
03604
152
195
03605
139
195
03606
138
180
Mean % Recovery
RSD of % Recovery
Giardia
% Recovered
78
71
77
75
5
Cysts
Reported
Cysts
Spiked
%
Recovered
100
91
0
201
201
0
50
45
N/A
48
7
Completed another set of PT samples in February 2007
OPT Recovery Statistics for Each Method Variation
Cryptosprodium Recoveries
Giardia Recoveries
Mean (%) Median (%) RSD (%) Mean (%) Median (%) RSD (%)
Method Version
# Labs
Envirochek
1
35
35
31
41
41
21
Envirochek HV
45
45
43
42
46
48
27
Filta-Max
15
47
46
35
39
43
44
Filta-Max Xpress
4
29
27
34
29
26
41
1
61
61
1
49
49
25
1
69
68
5
62
62
5
CrypTest
1
59
65
23
5
5
141
All Versions
68
45
44
41
43
46
35
5
48
Direct
Centrifugation
Continuous Flow
Centrifugation
WATERS Lab
75
7
Mean Recovery 52.4%
Sample Identification
Ongoing Precision and Recovery
07038-02
07025-02
07011-02
06362-02
06348-02
06334-02
06320-02
06306-02
06292-02
06278-02
06264-02
06251-02
06247-02
Percent Recovery
Cryptosporidium OPR
1.0
0.8
0.6
0.4
0.2
0.0
Mean Recovery 41.1%
Sample Identification
Ongoing Precision and Recovery
07038-02
07025-02
07011-02
06362-02
06348-02
06334-02
06320-02
06306-02
06292-02
06278-02
06264-02
06251-02
06247-02
Percent Recovery
Giardia OPR
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0.0
Education & Outreach
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2 Hour Crypto Presentation
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Approved for Drinking Water Operator CEU
Presented at KRWA Training Sessions
Will Present at KWWOA Annual Conference
1 Hour E. coli Presentation
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Approved for Waste Water Operator CEU
Presented at KRWA Training Sessions
Will Present for KWWOA
Environmental
Biotechnology
Rick Fowler
DNA Analysis Services
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DNA extraction from water, soil, membrane
filters, pure cultures, swabs, etc.
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Quantitative Real-Time PCR
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Yield of total DNA measured with Qubit fluorometer,
sensitivity 0.2 x 10-12 g
Determines concentration of DNA from specific
organisms in a mixture, sensitivity <1 ppt
DNA cloning and sequencing
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Cloning permits segregation of individual species
comprising environmental microbial communities
DNA sequencing yields unique gene sequences for
identification of organisms
Bacteria
Fungi
Amoebae
Parasites
Algae
Zooplankton
Field work in Mammoth Cave
Limestone
Ca13CO3
Examples of clients supported
by WATERS DNA services
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Louisville Water Company
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KSU Aquaculture Research Center
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Ammonia Oxidizing Bacteria in distribution system
Acanthamoeba in eyewash stations
DNA analysis of bacteria, algae, and fungi used to
evaluate conditions for optimal shrimp production
Fungi in some tanks identified by cloning and DNA
sequencing to assess potential risk or benefit
National Cave and Karst Research Institute
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Develop a universal monitoring scheme applicable
to caves nationwide and catalog any new
microorganisms indigenous to cave climates
Examples, continued
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Mammoth Cave National Park
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Ongoing monitoring of bacterial communities for
better understanding of karst aquifers and as
indicators of environmental changes
Monitor Chlorella DNA in surface waters to assist
with protection of endangered mussells
Oregon Caves National Monument
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Cloning and sequencing of bacterial and fungal
communities on- and off-tourist trails to assess
environmental impacts of tourism
Abundance of iron-sulfur bacteria indicate growth in
absence of light is derived from chemical energy
Scientific Outreach,
Presentations, and Workshops
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National Speleological Society
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National Cave and Karst Research Institute
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2001, 2004
National Park Service advisory panels
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2001, 2005
Geological Society of America
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2001, 2006
2001, 2002, 2003, 2004, 2005, 2006
ISEIS
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2006