Transcript Anheuser-Busch Training Presentation

ATP vs. Plate Counts
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Bioluminescence
Checks for microbial and
residual contamination
Results in less than 1 minute
Simple to use, no technical
expertise required
Allows you to check cleaning
practices before production,
avoiding costly contamination
problems
Provides speedy results
required by HACCP
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Plate Counts
Checks only for the specific
bacteria that can grow in the
selected medium.
Results in 24 to 48 hours
Requires technical lab support
Often requires you to throw
away product after
contamination results come
back
Do not provide the speed and
simplicity of feedback required
by modern HACCP demands.
Both have value but provide different results.
Relative ATP concentrations
Bacteria
Yeast
Somatic
1
100
300,000
ATP Compared to Traditional
Tests
5 organisms
5 units ATP
5 CFU
15 organisms
15 units ATP
3 CFU
20 organisms
20 units ATP
1 CFU
CFU to RLU Correlation
Residual ATP
CFU
Microbial ATP
RLU
RLU correlates well to CFU with
pure culture
Correlation does not occur because in
real world environment there is residual
ATP as well as microbial ATP.