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Bacterial Infection of Wound By Dr. Humodi A. Saeed Associate Prof. of Medical Microbiology College of Medical Laboratory Science Sudan University of Science and Technology E mail address: [email protected] Bacterial Infection of Wound The Wound Definition A wound is a breach (damage) in a normal tissue (May be superficial or deep) resulting accidentally or of planned surgery. The Wound Types 1. Early acute wound 2. Long-term chronic wounds Bacterial Infection of Wound • Wound contamination • Wound colonization • Wound Infection Bacterial Infection of Wound • Wound contamination • The presence of nonreplicating bacteria in the wound. Bacterial Infection of Wound Wound contamination • All chronic wounds are contaminated by bacteria. Bacterial Infection of Wound • Wound contamination • contaminants come from the indigenous microflora and/or the environment. Bacterial Infection Wound • Wound colonization: • The presence of replicating bacteria adherent to the wound in the absence of injury to the host. Bacterial Infection of Wound • Wound colonization: Most of the following organisms are normal skin flora. 1. S. epidermidis, 2. other coagulase –ve Staphylococci. 3. Corynebacterium sp. 4. Brevibacterium sp. 5. Proprionibacterium acnes Bacterial Infection of Wound Wound Infection The presence of replicating bacteria within a wound that cause host injury. Bacterial Infection of Wound Wound Infection 1. Staphylococcus aureus, 2. Beta-hemolytic Streptococcus (S. pyogenes, S. agalactiae) 3. E. coli 4. Proteus 5. Klebsiella 6. Anaerobes 7. Pseudomonas 8. Acinetobacter . Bacterial Infection of Wound Lab. Diagnosis specimen collection. 1. Tissue biopsy 2. Needle aspirate 3. Wound swab (Surface or Deep wound) Bacterial Infection of Wound Lab. Diagnosis • Suspected organism Bite wounds Eikenella corrodens, Pasteurella multocida, Pasteurella canis, Staphylococcus aureus, group A Streptococcus, mixed anaerobe, many gram-negative bacilli. • Surgical wounds Staphylococr:us aureus, coagulase-negative Staphylococcus, groups A and B Streptococci, Clostridium perfringens, Corynebacterium species many other bacteria. • Traumatic wounds Bacillus species, Staphylococcus aureus, group A Strepiococcus, many gram negative bacilli. Thank you I would like to thank Dr. Neal R. Chamberlain