Transcript 实验课件3

化脓菌分离鉴定过程（三）
化脓菌分离鉴定过程
葡萄球菌
• 甘露醇发酵试验
• 血浆凝固酶试验
• 耐热核酸酶试验
• 动物试验
甘露醇发酵试验
Inoculate the bacteria into a mannitol
micro-tube
Incubate at 37℃ for 18h.
S.aureus will ferment mannitol to
produce acid, which causes the medium
to turn yellow.
血浆凝固酶试验（The Coagulase Test）
 Coagulase
• 结合凝固酶
• 游离凝固酶
• 凝固酶
协同因子
纤维蛋白原
凝血酶样物质
纤维蛋白
 It has been speculated that this enzyme might be a
virulence factor. However, coagulase-negative
strains are often as pathogenic as coagulasepositive strains.
 There are two ways of performing this test
• The Slide Coagulase Test
• The Tube coagulase Test
Tube coagulase Test
＋
－
The Slide Coagulase Test
细菌的药物敏感性试验
纸片扩散法（K-B法）
肠道菌分离鉴定过程（三）
Antigens of salmonella
O antigen
H antigen
Vi antigen
polysaccharide of LPS
used to divide Salmonella to 42 groups
（A－Z，O51－63，O65－67）
A-E groups are pathogenic
flagella antigen
divide Salmonella tow phase:
special no special
related to the virulence of Salmonella
sensitive to heat ( lose activity at 60℃)
Serological Identification of Salmonella
Select the specimen
React with A-Z polyvalent antiserum
Agglutination
reaction
(＋)
React with several individual
types of anti-O and anti-H
antiserum
Identify its group and phage
(－)
React with anti-Vi
antiserum
系统实验报告（报告三）
化脓菌/肠道菌分离鉴定过程
目的
策略（原理设计）
方法步骤：简单写
结果（判定方法）
结论