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Biocontrol bacteria and actinomycetes, evaluation of their secondary metabolites against fungal pathogens and insectpest of chickpea Meesala Sree Vidya1 and S Gopalakrishnan2 1-Jawaharlal Nehru Technological University, Hyderabad; 2- International Crops Research Institute for the Semi Arid Tropics, Patancheru Different bacterial isolates inhibiting fungal pathogens. Introduction •It is well known that bacteria and actinomycetes found most common in compost and rhizosphere soils. Hence five different herbal vermicompost such as Neem, Annona, Gliricidia, Jatropha, Parthenium and rhizosphere soils from organic fields were selected for isolating the microbes. •Bacteria and actinomycetes isolated from above sources were characterized for their antagonistic activity against fugal pathogens and insect pests of chickpea. Most potential cultures will be further characterized for in-vitro PGP and biocontrol traits. S. rolfsii Vs VBI-1 Rb-6 Vs SBI-20 No of actinomycetes isolates antogonistic to different fungal pathogens Antogonistic Actinomycetes to Fungal pathogens 80 70 60 50 40 30 20 10 0 MP Rb-6 Objective Rb-24 Rb-115 Sclerotium FOC FM242 FM943 SRI CONT Fungal Pathogens The main objective of the present investigation is to isolate and identify the active compounds responsible for the inhibition of the fungal pathogens and H. armigera from the potential antagonistic microbes and entomopathogens Charcoal rot No of bacterial isolates antogonistic to different fungal pathogens 70 Antogonistic Bacteria to Fungal pathogens 60 No of Isolates Various fungal diseases of Chickpea Fusarium wilt FOC Vs SBI-20 FM-943 Vs SBI-20 No of Isolates •In the present investigation antagonistic and entomopathogenic bacteria and actinomycetes against important fungal pathogens Sclerotium rolfsii, Fusarium oxysporum f. sp ciceri (FOC), Rhizoctonia bataticola (3 strains, RB-6, -24 and -115), Fusarium andiyazi (FM943), Fusarium proliferatum (FM-242), Macrophomina phaseolina (MP) and insectpest of chickpea, Helicoverpa armigera, were studied. 50 40 30 20 10 0 MP Rb-6 Rb-24 Rb-115 Sclerotium FOC FM242 FM943 Fungal Pathogens Botrytis gray mold Dry root rot Results and Discussion •Out of 88 actinomycetes isolated, 55, 45, 58 and 37 isolates inhibited MP, Rb6, Rb-24 and Rb-115 respectively; 33, 36, 13 and 13 isolates inhibited FOC, FM242, FM-943 and S. rolfsii respectively. •From 72 bacteria isolated, 28, 25, 29 and 10 isolates inhibited MP, Rb-6, Rb24 and Rb-115 respectively; 31, 31, 5 and 22 isolates inhibited FOC, FM-242, FM-943 and S. rolfsii respectively. Materials and methods •Bacteria and actinomycetes were isolated from five herbal vermicomposts and rhizosphere soil from organic field by serial dilution method. •Antagonistic activity of the cultures were evaluated by dual culture assay. •The promising antagonistic cultures were further characterized for their PGP and biochemical traits. •The most promising isolates (20) were further evaluated for their PGP and biocontrol traits. The results indicated that xx, xx and xx were shown positive for chitinase, protease, lipase, respectively. Future work •Purification of active compound (s) from the culture filtrates. •Physiological characteristics of the promising cultures. •Green house and field evaluation of the promising cultures and their metabolites. *Corresponding author M. Sree Vidya, Email: [email protected]