Transcript Malignant Melanoma

BioLume
Presentation
January 10, 2007
BioLume
BioLume Mission
…..to develop and commercialize
a broad library of proprietary
bioluminescent proteins with
multiple first-in-class applications
in diagnostic imaging, food,
beverage, and cosmetics…..
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BioLume Imaging Patents
• Detection and visualization of neoplastic
tissues and other tissues
–U.S. 6,416,960 issued July 9, 2002
–U.S. 6,596,257 issued July 22, 2003
–U.S. 09/746485 filed December 22, 2000
• Broad claims e.g., “A diagnostic system
containing a bioluminescence generating
system”
• Backed up by broad composition of matter
patents
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What is Bioluminescence?
Bioluminescence is light produced
by a chemical reaction within a
living organism
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Bioluminescent Proteins
OXYGEN
o
COELENTERAZINE (Z)
+
ENZYME
"LUCIFERASE"
+
COMPLEX
o o
o o
c +
CO2
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Visible light
Bioluminescent Proteins
BioLume’s bioluminescent
proteins were cloned from various
deep-sea marine organisms
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Bioluminescent Proteins
Sardines, Herring, Haddock, Tuna, Salmon, and
Mackerel all have diets rich in bioluminescent
organisms and contain luciferases and their
substrate coelenterazine
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Bioluminescent Proteins
Food squid observed during fishery collection
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Bioluminescent Protein Safety
These proteins have already been tested for skin, eye,
and oral toxicity (both acute and sub-acute)
These proteins were negative for mutagenicity in the
Ames test.
Many pharmaceutical and university research laboratories
have maintained animals expressing Luciferase, with no
reports of toxicity or failure of animals or cells to thrive due
to their presence.
Coelenterazine is a natural antioxidant
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Molecular Imaging
Molecular imaging represents a paradigm
shift in the focus of medical imaging from
the visualization of anatomy and
physiology to the study of metabolic and
physiological processes e.g., cancers,
often in real time
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Molecular Imaging
Recent advances in biotechnology have
enabled in vivo molecular imaging of
luciferase reporter proteins in living animals
by using a cooled charge-coupled device
(CCD) camera.
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Bioluminescent Imaging
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Molecular Imaging
Because bioluminescence imaging
has minimal background activity, this
technology is very sensitive for
detecting light emitted from luciferases
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Bioluminescent Imaging
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Bioluminescence Molecular
Imaging with renilla luciferase
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Bioluminescent Imaging
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Bioluminescent Imaging
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Bioluminescent Imaging
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Bioluminescent Imaging
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Skin Cancer Facts
• >1 million new cases of skin cancer will be
diagnosed in the US this year (>10k will die)
• 1 in 5 Americans will develop skin cancer during
their lifetime (BCC, SCC, or melanoma)
• 1 in 65 Americans were likely to develop
melanoma during their lifetime
• Melanoma incidence has increased 690% since
1950
• Melanoma is the second most common cancer
in women aged 20 to 29
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Source: American Academy of Dermatology
Skin Cancer Detection
• Screening for skin cancer is a visual exam
by patient or dermatologist
• Suspicious lesions get a biopsy
• Highly specific antibodies for melanoma
are known and generically available
• There are no skin cancer screening kits
on the market today
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Immunology of Melanoma


There are unique surface antigens which
characterize melanoma cells
These unique antigens can be used to
target highly specific antibodies, for
therapy or detection
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Immunology of Melanoma
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
One type of surface antigen is a ganglioside (GM3)
Antibodies have been made which are directed against
these gangliosides as well as other melanoma surface
antigens
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Selective melanoma antibody
kill melanoma cells
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Bioluminescent
Melanoma Detection
Luciferases can be expressed with antibodies to
melanoma surface antigen(s)
Or
Luciferases can be chemically conjugated to the
melanoma antibodies
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Summary
• Product development opportunity combining
known antibodies and developed bioluminescent
proteins (no research required)
• Patented bioluminescent proteins
• Focused on an unmet medical need
• Large market opportunity
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