Transcript T cell mediated effects - UCSF Immunology Program

Autoimmunity
Mark S. Anderson, MD, PhD
University of California San Francisco
Autoimmunity
• Definition: immune response against self (auto-)
antigen
• General principles:
– Significant health burden, 5% of population
– Multiple factors contribute to autoimmunity,
including genetic predisposition, infections
– Fundamental problem is the failure of selftolerance
• Problems:
– Failure to identify target antigens, heterogeneous
disease manifestations, disease usually presents
long after initiation
Classification of Autoimmune
Diseases
• Broadly separated by the type of effector
mechanism (similar to hypersensitivity
classification scheme)
• Three classes:
– Type II:
Antibody against cell-surface
antigen or matrix antigens
– Type III:
Immune-complex disease
– Type IV:
T cell-mediated disease
Type II: AntibodyFigure
11-1 diseases
part 1 of
mediated
3
Graves’ disease
Figure 11-5
Graves’ disease:
Figure
11-7
Proof that it’s antibody
mediated
Myasthenia Gravis
In this disease, autoantibodies to the
Acetylcholine receptor block
neuromuscular transmission from
cholinergic neurons by blocking the
binding of acetylcholine and by
causing downregulation (degradation)
of its' receptor.
Type III: ImmuneFigure 11-1 part 2 of
complex mediated
diseases
3
Review: Immune
Figureformation
10-32
complex
Figure 11-10
A model for the
pathogenesis of
SLE
SLE: Immune complexes
Figure
13-33
in the kidney
Figure
part 3 of 3
Type IV:11-1
T cell-mediated
diseases
T cell mediated effects
(cellular immune)
– Direct T cell cytotoxicity via CD8+
CTL
– Self-destruction of tissue cells
induced by cytokines, eg, TNFa
– Recruitment and activation of
macrophages leading to bystander
tissue destruction
– Induction of target tissue apoptosis
by the T cell membrane protein FasL
Type
I Diabetes:
Figure
11-8 a T
cell-directed attack
against the b-cells of
the pancreatic islet
Type I Diabetes
• T cell response to antigens expressed in
the b-cells of the islets
– Proinsulin/Insulin, GAD, I-A2
– T cell response is Th1 “like”, makes g-IFN
and helps recruit a tissue/cell destruction
response
• >90% islet destruction needed for the
disease to be expressed
• Patients also have autoantibodies to
islet antigens
Tetramers: flow studies on PBMC
DR0401-MOG
DR0401-Control
DR3-proIns
DR3-Control
CD25
Tetramer
DR401-IGRP
DR401-control
DR404-GAD-555
CD4
DR0402-desmoglien
DR404-control
Tetramer
DR0402-control
DRB4-GAD557I
14.fcs
DRB4-control
12.fcs
7.28
%
100
101 102 103
CD4 APC-A
104
0.10
%
100
101 102 103
CD4 APC-A
104
4
Why do autoimmune diseases
occur?
Answer: Failure in T cell
tolerance
Mechanisms
of immune
Figure
13-16tolerance
Overview of Autoimmunity
Failure of central
or peripheral
tolerance
Genetic Predisposition
CD4+ T Cell
Driving Force
Specialized cells
present self-tissue
proteins
Environmental
Factors
Autoreactive
B Cells
IFN-gamma
IL-2, etc.
CD8+ T Cell
Driving Force
Tissue injury;
release of self antigens;
activation of self-reactive
lymphocytes
Autoantibodies
Because Autommunity is so
complex, how can we figure out
how it happens?
Answer:
1) Use genetics
2) Animal models
Genetic basis of autoimmunity
• Genetic predisposition of autoimmune diseases
– Increased incidence in twins
– Identification of disease-associated genes by breeding and
genomic approaches
• Multiple genes are associated with autoimmunity
– No single mutation causes autoimmunity
• MHC genes
– Major genetic association with autoimmune diseases (relative
risk)
– Disease-associated alleles may be found in normal individuals
• Non-MHC genes
– Many loci identified by genomic methods, animal studies
– Mutations in complement genes predispose to lupus
HLA (or MHC) is the strongest
genetic factor for susceptibility
to autoimmune disease
Figure 13-21
How does MHC predispose?
How do you find non-MHC
genes with weak effects?
GWAS (Genome Wide
Association Study)
SNP-Chip (an array of over
500,000 SNP’s!)
Genetics of autoimmunity: recent
successes of genomics
• NOD2: polymorphism associated with ~25% of
Crohn’s disease
– Microbial sensor in intestinal epithelial cells
• PTPN22: commonest autoimmunity-associated
gene; polymorphism in RA, SLE, others
– Phosphatase; mechanism of action?
• CD25 (IL-2R): associated with MS, others;
genome-wide association mapping
– Role in Tregs or effector cells?
• ATG16: autophagy gene
– Role of autophagy in IBD (resistance to microbes?)
• IL-23R: receptor for Th17-inducing cytokine
– Effect on Th17 responses
Informative single-gene models of
autoimmunity
• Fas/FasL (ALPS): peripheral deletion of T
and B cells
• FoxP3 (IPEX): Treg
• CTLA-4 (mouse KO): anergy; Treg
• IL-2, IL-2Rb (mouse KO): Treg
• Many others reported
BUT: not the basis of most autoimmune
diseases
Hereditary
C1q
deficiency
SPENCDI
AGS
ALPS
IPEX
APS1
Gene(s)
C1qA, C1qB, C1qC
TRAP (ACP5)
TREX1, RNaseH2
H2 (A, B, C),
SAMHD1
FAS, FASLG,
CASP10
FOXP3
AIRE
Inheritance
Autosomal recessive
Autosomal recessive
Autosomal recessive
Autosomal
dominant, autosomal
recessive, variable
penetrance
X-linked
Autosomal
recessive*
Main features
SLE and SLE-like
disease
Recurrent bacterial
infections
Skeletal dysplasia
Cerebral
calcifications and
CNS symptoms
SLE
Basal ganglia
calcifications,
neurologic
dysfunction, SLE
Lymphoproliferation
(lymphadenopathy
and/or
splenomegaly)
Autoimmune
cytopenias
Malignancy
Autoimmune
enteropathy
Neonatal diabetes
Thyroiditis
Eczema
Hypoparathyroidism
Adrenal
insufficiency
(Addison's disease)
Mucocutaneous
candidiasis
Autoimmunity
Systemic
Systemic
Systemic
Systemic, organspecific
Systemic, organspecific
Organ-specific
Autoimmune
features
SLE,
glomerulonephritis,
angioedema ,
+ANAs, +RNP Abs
SLE,
thrombocytopenia,
hemolytic anemia
SLE, chilblains,
hemolytic anemia,
+ANAs
Autoimmune
cytopenias
(hemolytic anemia,
thrombocytopenia,
neutropenia)
Enteropathy
Type 1 diabetes
Multi-organ disease
+Organ-specific
autoAbs
anti-IFN Abs,
NALP5 Abs
Tolerance
defect
Impaired clearance
of apoptotic material
Activation of Ttype
1 interferon signaling
Activation of type 1
interferon signaling
Defective
lymphocyte
apoptosis
Loss of Tregs
Defective deletional
tolerance
Central versus
peripheral
tolerance
mechanism
Peripheral
Peripheral
Peripheral
Peripheral
Peripheral
Central, ?peripheral?
Innate versus
adapative
immune defect
Innate
Innate
Innate
Adaptive
Adaptive
Adaptive
Immunodeficien
cy
Susceptibility to
encapsulated bacteria
None described
None described
Not generally
described
Recurrent infections
Candidiasis
Animal models of autoimmunity
• NOD mouse- model of type 1 diabetes
• NZBxNZW mouse-model of Lupus
• KBxN mouse-model of rheumatoid
arthritis
• EAE- induced model of multiple
sclerosis whereby disease is induced by
injecting proteins of the myelin sheath
with adjuvant
• Knockouts that get autoimmunity
Figure 13-3
Recent work in this model suggests
Th17 cells are important!
Figure 13-17
NOD mouse
spontaneously
gets diabetes
Figure 13-34
B7.1/B7.2 KO’s get worse diabetes
in the NOD background?
Answer is Treg’s, need B7’s to
generate Treg’s effectively
Forward genetics to find
autoimmune disease genes
Christopher C. Goodnow, Australia
ENU screen finds a line with
autoantibodies, glomerulonephritis, and
splenomegaly
Mice have increased germinal centers and
a defect in a gene (Sanroque) that
represses follicular T cells
What triggers autoimmune
disease?
Infections and autoimmunity
• Infections trigger autoimmune reactions
– Clinical prodromes, animal models
– Autoimmunity develops after infection is
eradicated (i.e. the autoimmune disease is
precipitated by infection but is not directly
caused by the infection)
• Some autoimmune diseases are reduced or prevented
by infections
– Increasing incidence of type 1 diabetes, multiple
sclerosis in developed countries; experimental NOD mice: mechanism unknown
– The “hygiene hypothesis” (originally proposed to
describe effects of infections on asthma)
Endocrine factors
• Most autoimmune disease do not occur with equal
frequency in males and females. For example Graves'
and Hashimoto's are 4-5 times, and SLE 10 times,
more common in females while Ankylosing Spondylitis
is 3-4 × more frequent in males. These differences
are believed to be the result of hormonal influences
• A second well documented hormonal effect is the
marked reduction in disease severity seen in many
autoimmune conditions during pregnancy. Rheumatoid
arthritis is perhaps the classic example of this
effect. In some cases there is also a rapid
exacerbation (rebound) after giving birth.
Microbes and autoimmunity?
Treatment
What would be the ideal way to treat
autoimmune disease?
Answer: remove only the antigenspecific response
Treatment (cont.)
Reality: Unable to remove the
antigen-specific response in general
Mainstay of treatment: antiinflammatories and global
immunosuppression if symptoms are
severe enough to warrant it
Therapeutic approaches for immune disorders
CTLA-4.Ig
(block costimulation)
Calcineurin, mTOR
inhibitors
(inhibit signaling)
CD28
IL-2
APC
TCR
IL-12, IL-23
(p40)
TNF, IL-1
TNF, IL-1
antagonists
Anti-p40
(block cytokines)
Inflammation
T cellAnti-IL-2R
(block cytokine
receptor)
IL-17A
Anti-IL-17A
Anti-integrin
antibodies
(block adhesion)
Therapeutics based on the B7:CD28/CTLA-4 family
1. Costimulatory blockade
CTLA-4.Ig is used for diseases caused by ….?
Therapeutics based on the B7:CD28/CTLA-4 family
1. Costimulatory blockade
CTLA-4.Ig is used for diseases caused by excessive
T cell activation -- rheumatoid arthritis, graft rejection; not yet approved for IBD,
psoriasis
Therapeutics based on the B7:CD28/CTLA-4 family
2. Inhibiting the inhibitor
Anti-CTLA-4 antibody is used for ….?
Therapeutics based on the B7:CD28/CTLA-4 family
2. Inhibiting the inhibitor
Anti-CTLA-4 antibody is approved for tumor
immunotherapy (enhancing immune responses against tumors)
Even more impressive early clinical trial results with anti-PD-1
in cancer patients
Type 1 Diabetes - A Disease of
the Immune System
Type 1 Diabetes is caused
by the autoimmune
destruction of insulin
producing ß-cells
TCR antigen
T Cell
T Cell
ß-cells
T-cells mediated killing of ß-cells
Progression in Type 1 Diabetes
Genetic
Predisposition
Environmental
Insult
100
AutoAbs
75
%Beta Cell
Mass
50
Abnormal IVGTT
25
Clinical Diagnosis
Honeymoon
10
Years
Checkpoints in the development
of autoimmune diabetes
Checkpoint 1
Insulitis
-Starts at weaning: immunological changes related to food uptake and
changes
in the intestinal flora
-Increased homing of T cells : expression of addressins MadCam and
PNAd
on pancreatic blood vessel epithelium
Checkpoint 2
Beta cell loss and diabetes
- T cells gain more aggressive effector mechanisms: Th1/Th2 balance,
cytokines,
Expression of Fas Ligand on CTLs
- Loss of protective mechanisms: Protective cytokines, Regulatory cells
- Amplification : Epitope spreading
Anti-CD3 mAb Treatment for
Autoimmunity
ISLETS
Suppression of autoimmunity with anti-CD3
DIABETES
Need to give at
disease onset!
Results of Herold anti-CD3 Phase I/II
trial in Type 1 Diabetes
Study Protocol
•New onset Type 1 diabetes mellitus in stable metabolic condition
•Within the first 6 weeks since diagnosis
•Age 8 – 35
•Two week single treatment with increasing doses of anti-CD3 mAbs
5 mg  4 mg/dose.
Drug tx
150
Control
*
*
100
*
50
0
0
6
12
Month
*p=0.003 vs control
18
24
Insulin dose (U/Kg)
AUC (pmol/ml/240min)
•23 treated patients and 23 control subjects undergoing metabolic studies over 2 years
1
0.8
**
**
**
0.6
0.4
0.2
0
0
6
12
Month
**p<0.02 vs control
18
24
Autoimmune diseases
• Animal models are revealing pathways of
immune regulation and why it fails
• Genetic studies are identifying underlying
defects in human diseases
• Analytical methods for human diseases are
improving
• Challenges:
– From genes to pathways (molecular and
functional)
– Using the knowledge to develop therapies