Transcript Further panels are available eg for inflammation

Gene Expression Profiling
Single molecule quantification of RNA molecules using nCounter SPRINT™ Technology from NanoString

Workflow
 Sample preparation (e.g. cells treated with different
compounds)
 Cell lysis
 Mixing samples with target gene specific capture and
reporter RNA probes
 Hybridization at 65°C over night
 Removal of non-hybridized RNA probes by nCounter
SPRINT fluidic system
 Single molecule quantification of captured RNA via
gene specific barcodes
 Advantages
 Up to 800 genes profiled in parallel
 Up to 36 samples/day
 Ultra high precision (as low as 10% change in
expression levels)
 Applicable with purified RNA and non-purified RNA
(cell lysates)
 Only 50ng RNA required (ideally 5ul 10 ng/µl)
 Pre-selected gene panels available (e.g. pan cancer,
pan cancer immune, pan cancer progression,
inflammation etc.)
0
 Easy generation of customized gene panels
provided from NanoString
Gene Expression Profiling
Single molecule quantification of RNA molecules using nCounter SPRINT™ Technology from NanoString

Ready available Sets of Reporter probe mRNA (code sets)
Oncology
 PanCancer Pathway Panel (770 genes)
 PanCancer Progression Panel (770 genes)
 Human PanCancer Immune Panel (770 genes)
 Mouse PanCancer Immune Panel (770 genes)
 Breast Cancer Estrogen Receptor Panel (200 genes)
Immunology
 Human Immunology Panel (594 genes)
 Mouse Immunology Panel (561 genes)
Further panels are available e.g. for inflammation
Please click on NanoString logo to view ready available code sets
provided from NanoString
1