Transcript pbi12108-sup-0001-FigS1

(a)
NOS-Ter GUS
35S-Pro
35S-Pro
NOS-Ter
OFP
(b)
Bright field
*
OFP filter
*
(c)
Figure S1. Demonstration of coexpression of two reporter genes in transgenic hairy roots of
soybean. (a) Schematic representation of the construct used for coexpression of an orange
fluorescent protein (OFP) reporter gene and a GUS reporter gene. “35S-Pro” and “NOS-ter”
represent the CaMV 35S promoter and the NOS terminator, respectively. (b) Analysis of
transgenic hairy roots with the construct described in (a). OFP-positive transgenic hairy root
(labeled with an asterisk) were separated from OFP-negative hairy root (labeled with a
triangle) under OFP filter. (c) OFP-positive hairy roots were further demonstrated to be
positive for GUS staining.